The MAL1 locus of Saccharomyces cerevisiae comprises three genes necessary for maltose utilization. They include regulatory, maltose transport and maltase genes designated MAL1R, MAL1T and MAL1S respectively. Using a MAL1 strain transformed with an episomal, multicopy plasmid carrying the MAL2 locus, five recessive and one dominant mutant unable to grow on maltose, but still retaining a functional MAL1 locus were isolated. All the mutants could use glycerol, ethanol, raffinose and sucrose as a sole carbon source; expression of the maltase and maltose permease genes was severely and coordinately reduced. Only the dominant mutant failed to accumulate the MAL1R mRNA

Vanoni, M., & Goldenthal, M. (1991). Isolation and characterization of maltose non utilizing (mnu) mutants mapping outside the MAL1 locus in Saccharomyces cerevisiae. FEMS MICROBIOLOGY LETTERS, 61(2-3), 233-236.

Isolation and characterization of maltose non utilizing (mnu) mutants mapping outside the MAL1 locus in Saccharomyces cerevisiae

VANONI, MARCO ERCOLE;
1991

Abstract

The MAL1 locus of Saccharomyces cerevisiae comprises three genes necessary for maltose utilization. They include regulatory, maltose transport and maltase genes designated MAL1R, MAL1T and MAL1S respectively. Using a MAL1 strain transformed with an episomal, multicopy plasmid carrying the MAL2 locus, five recessive and one dominant mutant unable to grow on maltose, but still retaining a functional MAL1 locus were isolated. All the mutants could use glycerol, ethanol, raffinose and sucrose as a sole carbon source; expression of the maltase and maltose permease genes was severely and coordinately reduced. Only the dominant mutant failed to accumulate the MAL1R mRNA
Articolo in rivista - Articolo scientifico
Scientifica
RNA, Messenger; Genotype; Blotting, Northern; Membrane Transport Proteins; Genes, Fungal; alpha-Glucosidases; RNA, Fungal; Mutation; Monosaccharide Transport Proteins; Saccharomyces cerevisiae; Maltose
English
Vanoni, M., & Goldenthal, M. (1991). Isolation and characterization of maltose non utilizing (mnu) mutants mapping outside the MAL1 locus in Saccharomyces cerevisiae. FEMS MICROBIOLOGY LETTERS, 61(2-3), 233-236.
Vanoni, M; Goldenthal, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/34320
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