The effects of temperature (in the range 15-36 degrees C) on growth and the nuclear and budding cycle have been studied in populations of the yeast Saccharomyces cerevisiae exponentially growing in batch on yeast nitrogen base (YNB) glucose medium. The maximal rate of exponential growth is achieved at 30 degrees C, and a transition point is apparent at about 20 degrees C. At all tested temperatures DNA replication begins when cells are still unbudded and both the budded period and the postreplicative period have the same temperature dependence. A temperature compensatory mechanism seems to operate in S phase, during which duration remains relatively constant, in the range 21-36 degrees C, while duration of G2+ M phases shows a much more pronounced temperature dependence. The results are discussed in terms of a cell-cycle model for budding yeast.
Vanoni, M., Vai, M., & Frascotti, G. (1984). Effects of temperature on the yeast cell cycle analyzed by flow cytometry. CYTOMETRY, 5(5), 530-533.
|Citazione:||Vanoni, M., Vai, M., & Frascotti, G. (1984). Effects of temperature on the yeast cell cycle analyzed by flow cytometry. CYTOMETRY, 5(5), 530-533.|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Titolo:||Effects of temperature on the yeast cell cycle analyzed by flow cytometry|
|Autori:||Vanoni, M; Vai, M; Frascotti, G|
|Data di pubblicazione:||1984|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/cyto.990050515|
|Appare nelle tipologie:||01 - Articolo su rivista|