DNA double-strand breaks (DSBs) can be repaired by non-homologous end-joining (NHEJ) or homologous recombination (HR). HR is initiated by nucleolytic degradation of the DSB ends in a process termed resection. The Mre11-Rad50-Xrs2/NBS1 (MRX/N) complex is a multifunctional enzyme that, aided by the Sae2/CtIP protein, promotes DSB resection and maintains the DSB ends tethered to each other to facilitate their re-ligation. Furthermore, it activates the protein kinase Tel1/ATM, which initiates DSB signaling. In Saccharomyces cerevisiae, these MRX functions are inhibited by the Rif2 protein, which is enriched at telomeres and protects telomeric DNA from being sensed and processed as a DSB. The present review focuses on recent data showing that Sae2 and Rif2 regulate MRX functions in opposite manners by interacting with Rad50 and influencing ATP-dependent Mre11-Rad50 conformational changes. As Sae2 is enriched at DSBs whereas Rif2 is predominantly present at telomeres, the relative abundance of these two MRX regulators can provide an effective mechanism to activate or inactivate MRX depending on the nature of chromosome ends.

Bonetti, D., Clerici, M., Longhese, M. (2021). Interplay between Sae2 and Rif2 in the regulation of Mre11-Rad50 activities at DNA ends. CURRENT OPINION IN GENETICS & DEVELOPMENT, 71(December 2021), 72-77 [10.1016/j.gde.2021.07.001].

Interplay between Sae2 and Rif2 in the regulation of Mre11-Rad50 activities at DNA ends

Bonetti D.
Primo
Membro del Collaboration Group
;
Clerici M.
Secondo
Membro del Collaboration Group
;
Longhese M. P.
Ultimo
Membro del Collaboration Group
2021

Abstract

DNA double-strand breaks (DSBs) can be repaired by non-homologous end-joining (NHEJ) or homologous recombination (HR). HR is initiated by nucleolytic degradation of the DSB ends in a process termed resection. The Mre11-Rad50-Xrs2/NBS1 (MRX/N) complex is a multifunctional enzyme that, aided by the Sae2/CtIP protein, promotes DSB resection and maintains the DSB ends tethered to each other to facilitate their re-ligation. Furthermore, it activates the protein kinase Tel1/ATM, which initiates DSB signaling. In Saccharomyces cerevisiae, these MRX functions are inhibited by the Rif2 protein, which is enriched at telomeres and protects telomeric DNA from being sensed and processed as a DSB. The present review focuses on recent data showing that Sae2 and Rif2 regulate MRX functions in opposite manners by interacting with Rad50 and influencing ATP-dependent Mre11-Rad50 conformational changes. As Sae2 is enriched at DSBs whereas Rif2 is predominantly present at telomeres, the relative abundance of these two MRX regulators can provide an effective mechanism to activate or inactivate MRX depending on the nature of chromosome ends.
Articolo in rivista - Review Essay
MRX/N, Sae2, Rif2, DNA ends;
English
72
77
6
Bonetti, D., Clerici, M., Longhese, M. (2021). Interplay between Sae2 and Rif2 in the regulation of Mre11-Rad50 activities at DNA ends. CURRENT OPINION IN GENETICS & DEVELOPMENT, 71(December 2021), 72-77 [10.1016/j.gde.2021.07.001].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/337718
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