The time decay of the fluorescence polarization anisotropy of calf thymus DNA-ethidium complexes is obtained from measurements with sine-modulated excitation employing the so-called multifrequency phase fluorometry. A torsional dynamics model developed by J. M. Schurr [ (1984) Chemical Physics, Vol. 84, pp. 71-96] and translated into the frequency domain is found here to describe accurately DNA-ethidium fluorescence data collected under modulated excitation. At a low dye /DNA ratio (1 : 400) the value of the DNA torsional constant (alpha = 4.63 +/- 0.2 10(-12) dyne cm) fitting the data agrees very well with the known values of alpha. When the measurements are extended to a higher ethidium/DNA ratio, energy transfer effects between intercalated dyes are observed. A theoretical prediction of the donor and acceptor dye contributions to the fluorescence polarization anisotropy is made here, taking into account also dye-dye distance distributions

Collini, M., Chirico, G., & Baldini, G. (1992). DNA torsional dynamics by multifrequency phase fluorometry. BIOPOLYMERS, 32(11), 1447-1459 [10.1002/bip.360321105].

DNA torsional dynamics by multifrequency phase fluorometry

COLLINI, MADDALENA;CHIRICO, GIUSEPPE;BALDINI, GIANCARLO
1992

Abstract

The time decay of the fluorescence polarization anisotropy of calf thymus DNA-ethidium complexes is obtained from measurements with sine-modulated excitation employing the so-called multifrequency phase fluorometry. A torsional dynamics model developed by J. M. Schurr [ (1984) Chemical Physics, Vol. 84, pp. 71-96] and translated into the frequency domain is found here to describe accurately DNA-ethidium fluorescence data collected under modulated excitation. At a low dye /DNA ratio (1 : 400) the value of the DNA torsional constant (alpha = 4.63 +/- 0.2 10(-12) dyne cm) fitting the data agrees very well with the known values of alpha. When the measurements are extended to a higher ethidium/DNA ratio, energy transfer effects between intercalated dyes are observed. A theoretical prediction of the donor and acceptor dye contributions to the fluorescence polarization anisotropy is made here, taking into account also dye-dye distance distributions
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Articolo in rivista - Articolo scientifico
Scientifica
torsional dynamics, multifrequency phase fluorometry
English
Collini, M., Chirico, G., & Baldini, G. (1992). DNA torsional dynamics by multifrequency phase fluorometry. BIOPOLYMERS, 32(11), 1447-1459 [10.1002/bip.360321105].
Collini, M; Chirico, G; Baldini, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/33712
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