The time decay of the fluorescence polarization anisotropy of calf thymus DNA-ethidium complexes is obtained from measurements with sine-modulated excitation employing the so-called multifrequency phase fluorometry. A torsional dynamics model developed by J. M. Schurr [ (1984) Chemical Physics, Vol. 84, pp. 71-96] and translated into the frequency domain is found here to describe accurately DNA-ethidium fluorescence data collected under modulated excitation. At a low dye /DNA ratio (1 : 400) the value of the DNA torsional constant (alpha = 4.63 +/- 0.2 10(-12) dyne cm) fitting the data agrees very well with the known values of alpha. When the measurements are extended to a higher ethidium/DNA ratio, energy transfer effects between intercalated dyes are observed. A theoretical prediction of the donor and acceptor dye contributions to the fluorescence polarization anisotropy is made here, taking into account also dye-dye distance distributions
Collini, M., Chirico, G., & Baldini, G. (1992). DNA torsional dynamics by multifrequency phase fluorometry. BIOPOLYMERS, 32(11), 1447-1459 [10.1002/bip.360321105].
Citazione: | Collini, M., Chirico, G., & Baldini, G. (1992). DNA torsional dynamics by multifrequency phase fluorometry. BIOPOLYMERS, 32(11), 1447-1459 [10.1002/bip.360321105]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | DNA torsional dynamics by multifrequency phase fluorometry | |
Autori: | Collini, M; Chirico, G; Baldini, G | |
Autori: | ||
Data di pubblicazione: | 1992 | |
Lingua: | English | |
Rivista: | BIOPOLYMERS | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/bip.360321105 | |
Appare nelle tipologie: | 01 - Articolo su rivista |