Ion-exchange high-performance liquid chromatography (HPLC) of the total extract of the pollen of Parietaria judaica allows the separation of several allergens in the acidic pI range and could be used for enrichment procedures. Prior treatment by size-exclusion HPLC improves the efficiency of the purification slightly, allowing further fractionation of a peak at about 12 500 daltons. Isoelectrofocusing and radio-allergo-sorbent test are used for the analysis of individual fractions.
Bolzacchini, E., Digregorio, G., Nali, M., Rindone, B., Tollari, S., Falagiani, P., et al. (1987). Combined size-exclusion ion-exchange high-performance liquid-chromatographic analysis of parietaria pollen extracts with the aid of isoelectrofocusing and a radio-allergo-sorbent test. JOURNAL OF CHROMATOGRAPHY A, 397(C), 299-306 [10.1016/S0021-9673(01)85012-3].
Combined size-exclusion ion-exchange high-performance liquid-chromatographic analysis of parietaria pollen extracts with the aid of isoelectrofocusing and a radio-allergo-sorbent test
BOLZACCHINI, EZIO;RINDONE, BRUNO;
1987
Abstract
Ion-exchange high-performance liquid chromatography (HPLC) of the total extract of the pollen of Parietaria judaica allows the separation of several allergens in the acidic pI range and could be used for enrichment procedures. Prior treatment by size-exclusion HPLC improves the efficiency of the purification slightly, allowing further fractionation of a peak at about 12 500 daltons. Isoelectrofocusing and radio-allergo-sorbent test are used for the analysis of individual fractions.
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