The understanding of human diseases has been greatly expanded thanks to the study of animal models. Nonetheless, histopathological evaluation of experimental models needs to be as rigorous as that applied for human samples. Indeed, drawing reliable and accurate conclusions is critically influenced by the quality of tissue section preparation. Here, we describe a protocol for histopathological analysis of murine tissues that implements several automated steps during the procedure, from the initial preparation to the paraffin embedding of the murine samples. The reduction of methodological variables through rigorous protocol standardization from automated procedures contributes to increased overall reliability of murine pathological analysis. Specifically, this protocol describes the utilization of automated processing and embedding robotic systems, routinely used for the tissue processing and paraffin embedding of human samples, to process murine specimens of intestinal inflammation. We conclude that the reliability of histopathological examination of murine tissues is significantly increased upon introduction of standardized and automated techniques.

Cribiu, F., Burrello, C., Tacchi, R., Boggio, F., Ricca, D., Caprioli, F., et al. (2019). Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses. JOURNAL OF VISUALIZED EXPERIMENTS, 2019(143) [10.3791/58654].

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Facciotti F
Ultimo
2019

Abstract

The understanding of human diseases has been greatly expanded thanks to the study of animal models. Nonetheless, histopathological evaluation of experimental models needs to be as rigorous as that applied for human samples. Indeed, drawing reliable and accurate conclusions is critically influenced by the quality of tissue section preparation. Here, we describe a protocol for histopathological analysis of murine tissues that implements several automated steps during the procedure, from the initial preparation to the paraffin embedding of the murine samples. The reduction of methodological variables through rigorous protocol standardization from automated procedures contributes to increased overall reliability of murine pathological analysis. Specifically, this protocol describes the utilization of automated processing and embedding robotic systems, routinely used for the tissue processing and paraffin embedding of human samples, to process murine specimens of intestinal inflammation. We conclude that the reliability of histopathological examination of murine tissues is significantly increased upon introduction of standardized and automated techniques.
Articolo in rivista - Articolo scientifico
robotic systems, histology, colitis, murine models, protocols
English
2019
2019
143
e58654
none
Cribiu, F., Burrello, C., Tacchi, R., Boggio, F., Ricca, D., Caprioli, F., et al. (2019). Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses. JOURNAL OF VISUALIZED EXPERIMENTS, 2019(143) [10.3791/58654].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/335353
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