Molecules of a green fluorescent protein mutant, GFPmut2, have been immobilized in nanocapsules, assemblies of charged polyelectrolyte multilayers, with the aim to study the effect of protein-polyelectrolyte interactions on the protein stability against chemical denaturation. GFPmut2 proteins turn out to be stabilized and protected against the denaturating action of small chemical compounds. The nanocapsule protective effect on GFPmut2 is likely due to protein interactions with the negatively charged polymers, that induce an increase in the local rigidity of the protein nano-environment. This suggestion is supported by Fluorescence Polarization measurements on GFPmut2 proteins bound to the NC layers

Krol, S., Cannone, F., Caccia, M., Sironi, L., Campanini, B., Collini, M., et al. (2008). Structural Stability of Green Fluorescent Proteins Entrapped in Polyelectrolyte Nanocapsules. JOURNAL OF BIOPHOTONICS, 1(4), 310-319 [10.1002/jbio.200810033].

Structural Stability of Green Fluorescent Proteins Entrapped in Polyelectrolyte Nanocapsules

SIRONI, LAURA;COLLINI, MADDALENA;CHIRICO, GIUSEPPE;
2008

Abstract

Molecules of a green fluorescent protein mutant, GFPmut2, have been immobilized in nanocapsules, assemblies of charged polyelectrolyte multilayers, with the aim to study the effect of protein-polyelectrolyte interactions on the protein stability against chemical denaturation. GFPmut2 proteins turn out to be stabilized and protected against the denaturating action of small chemical compounds. The nanocapsule protective effect on GFPmut2 is likely due to protein interactions with the negatively charged polymers, that induce an increase in the local rigidity of the protein nano-environment. This suggestion is supported by Fluorescence Polarization measurements on GFPmut2 proteins bound to the NC layers
Si
Articolo in rivista - Articolo scientifico
Scientifica
Nanocapsules, GFP, fluorescence
English
310
319
10
Krol, S., Cannone, F., Caccia, M., Sironi, L., Campanini, B., Collini, M., et al. (2008). Structural Stability of Green Fluorescent Proteins Entrapped in Polyelectrolyte Nanocapsules. JOURNAL OF BIOPHOTONICS, 1(4), 310-319 [10.1002/jbio.200810033].
Krol, S; Cannone, F; Caccia, M; Sironi, L; Campanini, B; Collini, M; Chirico, G; Diaspro, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/3321
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