Background: Inflammation and excitotoxicity play important roles in the pathophysiology of ischemic brain damage. In previous studies we have shown a prolonged TNF-alpha (TNF-a) release, a long lasting high glutamate (glu) plasma concentration and an impaired platelet (plt) glu transport up to 3 months after the symptoms onset. Since high persisting plasmatic levels of TNF-a have been detected in stroke patients, we aimed to determine whether TNF-a administration could be sufficient to cause a defective plt glutamate uptake. Methods: Human blood plt were selected as a peripheral model of glutamate transport. 10 stroke patients and 20 control subjects were enrolled to test [H3]glu uptake after stimulation with increasing doses of TNF-a (10, 20 and 40ng/ml). Results: Plt from healthy subjects displayed a dose related reduction of glu uptake after TNF-a stimulation (total glu uptake decrease -35%). The reduction was less extensive in plt from stroke patients, possibly due to the demonstrated higher levels of plasmatic TNF-a in patients affected by stroke. Discussion: We showed that in-vitro administration of high concentration of TNF-a is able to effectively reduce glu uptake in human plt. The results suggest that the increase in plasmatic TNF-a in stroke patients may be responsible for the demonstrated reduction of plt glu uptake. Since decreased plt glu uptake could result in increased plasmatic glu levels, which in turn may generate a possible excitotoxic damage, pharmacological modulation of TNF-a levels might be helpful in the management of cerebrovascular patients.
Piazza, F., Marco, L., Ivan, G., & Ferrarese, C. (2007). Effect of TNF-alpha on platelet glutamate uptake in Stroke patients. CEREBROVASCULAR DISEASES, 23(S2), 63-63.
|Citazione:||Piazza, F., Marco, L., Ivan, G., & Ferrarese, C. (2007). Effect of TNF-alpha on platelet glutamate uptake in Stroke patients. CEREBROVASCULAR DISEASES, 23(S2), 63-63.|
|Tipo:||Abstract in rivista|
|Carattere della pubblicazione:||Scientifica|
|Presenza di un coautore afferente ad Istituzioni straniere:||No|
|Titolo:||Effect of TNF-alpha on platelet glutamate uptake in Stroke patients|
|Autori:||Piazza, F; Marco, L; Ivan, G; Ferrarese, C|
PIAZZA, FABRIZIO (Primo)
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1159/000103854|
|Appare nelle tipologie:||01 - Articolo su rivista|