To achieve growth, microbial organisms must cope with stresses and adapt to the envi- ronment, exploiting the available nutrients with the highest efficiency. In Saccharomyces cerevisiae, Ras/PKA and Snf1/AMPK pathways regulate cellular metabolism according to the supply of glucose, alternatively supporting fermentation or mitochondrial respiration. Many reports have highlighted crosstalk between these two pathways, even without providing a comprehensive mechanism of regulation. Here, we show that glucose-dependent inactivation of Snf1/AMPK is independent from the Ras/PKA pathway. Decoupling glucose uptake rate from glucose concentration, we highlight a strong coordination between glycolytic metabolism and Snf1/AMPK, with an inverse correlation between Snf1/AMPK phosphorylation state and glucose uptake rate, regardless of glucose concentra- tion in the medium. Despite fructose-1,6-bisphosphate (F1,6BP) being proposed as a glycolytic flux sensor, we demonstrate that glucose-6-phosphate (G6P), and not F1,6BP, is involved in the control of Snf1/AMPK phosphorylation state. Altogether, this study supports a model by which Snf1/AMPK senses glucose flux independently from PKA activity, and thanks to conversion of glucose into G6P.
Milanesi, R., Tripodi, F., Vertemara, J., Tisi, R., & Coccetti, P. (2021). AMPK Phosphorylation Is Controlled by Glucose Transport Rate in a PKA-Independent Manner. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(17) [10.3390/ijms22179483].
Citazione: | Milanesi, R., Tripodi, F., Vertemara, J., Tisi, R., & Coccetti, P. (2021). AMPK Phosphorylation Is Controlled by Glucose Transport Rate in a PKA-Independent Manner. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(17) [10.3390/ijms22179483]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | AMPK Phosphorylation Is Controlled by Glucose Transport Rate in a PKA-Independent Manner | |
Autori: | Milanesi, R; Tripodi, F; Vertemara, J; Tisi, R; Coccetti, P | |
Autori: | ||
Data di pubblicazione: | 2021 | |
Lingua: | English | |
Rivista: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.3390/ijms22179483 | |
Appare nelle tipologie: | 01 - Articolo su rivista |