Background: Peripheral neuropathy treatment is not always satisfactory. To fill this gap, inferences from bench side are warranted, where morphological and pathogenetic determinations can be performed. Nerve conduction studies (NCS) are ideal to translate results from preclinical to clinical setting. New methods: We propose a comprehensive 8-minute protocol for sensory-motor neurophysiological assessment, similar to routine clinical practice: sensory proximal and distal caudal nerves, motor caudal nerve, and sensory digital nerve recordings were used and tested in 2 different experimental settings. In Experiment 1 we compared control (CTRL) animals to a severe sensory-motor polyneuropathy (animals treated with vincristine [VCR]), and in Experiment 2 CTRL animals were compared to a mild sensory polyneuropathy (animals treated with oxaliplatin [OHP]). NCS were performed after 1-month of chemotherapy and matched with confirmatory neuropathological analyses. Results: VCR treated animals showed, at NCS, a relevant sensory-motor polyneuropathy ensued at the end of treatment; whereas, OHP animals showed a mild distal sensory neuropathy. These patterns were confirmed by neuropathological analysis. Comparison with existing methods: In literature, the majority of proposed neurophysiological protocols relies mainly on a single nerve testing, rather than a combination of them, and only a few studies tested both caudal and sciatic nerve branches, nevertheless not aiming at fully reproduce clinical protocols (e.g., seeking for length-dependency); to provide evidence of appropriateness of our protocol we applied a gold standard: neuropathology. Conclusion: The simple and rapid protocol here presented can be suggested as a good translation outcome measure in preclinical setting.

Monza, L., Fumagalli, G., Chiorazzi, A., Alberti, P. (2021). Translating morphology from bench side to bed side via neurophysiology: 8-minute protocol for peripheral neuropathy research. JOURNAL OF NEUROSCIENCE METHODS, 363(1 November 2021), 1-6 [10.1016/j.jneumeth.2021.109323].

Translating morphology from bench side to bed side via neurophysiology: 8-minute protocol for peripheral neuropathy research

Monza, Laura
Primo
;
Fumagalli, Giulia
Secondo
;
Chiorazzi, Alessia
Penultimo
;
Alberti, Paola
Ultimo
2021

Abstract

Background: Peripheral neuropathy treatment is not always satisfactory. To fill this gap, inferences from bench side are warranted, where morphological and pathogenetic determinations can be performed. Nerve conduction studies (NCS) are ideal to translate results from preclinical to clinical setting. New methods: We propose a comprehensive 8-minute protocol for sensory-motor neurophysiological assessment, similar to routine clinical practice: sensory proximal and distal caudal nerves, motor caudal nerve, and sensory digital nerve recordings were used and tested in 2 different experimental settings. In Experiment 1 we compared control (CTRL) animals to a severe sensory-motor polyneuropathy (animals treated with vincristine [VCR]), and in Experiment 2 CTRL animals were compared to a mild sensory polyneuropathy (animals treated with oxaliplatin [OHP]). NCS were performed after 1-month of chemotherapy and matched with confirmatory neuropathological analyses. Results: VCR treated animals showed, at NCS, a relevant sensory-motor polyneuropathy ensued at the end of treatment; whereas, OHP animals showed a mild distal sensory neuropathy. These patterns were confirmed by neuropathological analysis. Comparison with existing methods: In literature, the majority of proposed neurophysiological protocols relies mainly on a single nerve testing, rather than a combination of them, and only a few studies tested both caudal and sciatic nerve branches, nevertheless not aiming at fully reproduce clinical protocols (e.g., seeking for length-dependency); to provide evidence of appropriateness of our protocol we applied a gold standard: neuropathology. Conclusion: The simple and rapid protocol here presented can be suggested as a good translation outcome measure in preclinical setting.
Articolo in rivista - Articolo scientifico
Animal models; Caudal nerve; Digital nerve; Motor and sensory nerve conduction studies; Neuropathy; Translational medicine;
English
13-ago-2021
2021
363
1 November 2021
1
6
109323
reserved
Monza, L., Fumagalli, G., Chiorazzi, A., Alberti, P. (2021). Translating morphology from bench side to bed side via neurophysiology: 8-minute protocol for peripheral neuropathy research. JOURNAL OF NEUROSCIENCE METHODS, 363(1 November 2021), 1-6 [10.1016/j.jneumeth.2021.109323].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/324169
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