Direct detection of paramagnetic species in control and adriamycin-perfused rat hearts has been carried out. Depending on the flow rate of the perfusion solution(8,4,2 and 1 ml/min) different paramagnetic species were observed: Fe(III)(g=2.12)at 4 ml/min; three types of oxygen centered radicals of which two in control hearts(g{norm of matrix}1=2.05 g{norm of matrix}2=2.038 g⊥1=g⊥2=2.008) and the third one (g{norm of matrix}=2.03 g⊥=2.005) in adriamycin perfused hearts, at 2 ml/min. The latter radical was the only one observed at perfusion rate of 1 ml/min both in control and adriamycin treated systems. A relationship between the intracellular enzymatic reductive activation of the anthracycline and the occurrence of ischemic conditions(4,2 and 1 ml/min) in myocardial tissues is proposed basing on the relative amounts of the paramagnetic species above mentioned. © 1988 Academic Press, Inc.
Costa, L., Malatesta, V., Morazzoni, F., Scotti, R., Monti, E., Paracchini, L. (1988). DIRECT DETECTION OF PARAMAGNETIC SPECIES IN ADRIAMYCIN PERFUSED RAT HEARTS. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 153(1), 275-280 [10.1016/S0006-291X(88)81218-X].
DIRECT DETECTION OF PARAMAGNETIC SPECIES IN ADRIAMYCIN PERFUSED RAT HEARTS
MORAZZONI, FRANCA;SCOTTI, ROBERTO;
1988
Abstract
Direct detection of paramagnetic species in control and adriamycin-perfused rat hearts has been carried out. Depending on the flow rate of the perfusion solution(8,4,2 and 1 ml/min) different paramagnetic species were observed: Fe(III)(g=2.12)at 4 ml/min; three types of oxygen centered radicals of which two in control hearts(g{norm of matrix}1=2.05 g{norm of matrix}2=2.038 g⊥1=g⊥2=2.008) and the third one (g{norm of matrix}=2.03 g⊥=2.005) in adriamycin perfused hearts, at 2 ml/min. The latter radical was the only one observed at perfusion rate of 1 ml/min both in control and adriamycin treated systems. A relationship between the intracellular enzymatic reductive activation of the anthracycline and the occurrence of ischemic conditions(4,2 and 1 ml/min) in myocardial tissues is proposed basing on the relative amounts of the paramagnetic species above mentioned. © 1988 Academic Press, Inc.
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