Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.

Christoffersen, S., Serra, I., Terreni, M., Piškur, J. (2010). Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine. NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS, 29(4-6), 445-448 [10.1080/15257771003741422].

Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine

Serra I.;
2010

Abstract

Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.
Articolo in rivista - Articolo scientifico
2',3'-dideoxyinosine; anti-viral drugs; biocatalysis; Clostridium perfringens; Nucleoside phosphorylase;
English
2010
29
4-6
445
448
none
Christoffersen, S., Serra, I., Terreni, M., Piškur, J. (2010). Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine. NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS, 29(4-6), 445-448 [10.1080/15257771003741422].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/320298
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