Intrinsically disordered proteins are being paid an increasing amount of interest due to the understanding of the crucial role that flexible regions play in molecular recognition and in signaling. Accordingly, reports focusing on the structural and functional characterization of intrinsically disordered proteins or regions are growing exponentially. Relatively few studies have however been reported on the mutual effects of ordered and disordered moieties in artificial fusion proteins. In this review, we focus on the few available experimental data based on the use of chimeras in which fluorescent proteins were fused to disordered domains of different lengths, compactness and propensity to form secondary structures. The impact of the artificial fusion on the conformational and functional properties of the resulting proteins is discussed.

Lotti, M., Longhi, S. (2012). Mutual effects of disorder and order in fusion proteins between intrinsically disordered domains and fluorescent proteins. MOLECULAR BIOSYSTEMS, 8(1), 105-113 [10.1039/c1mb05244f].

Mutual effects of disorder and order in fusion proteins between intrinsically disordered domains and fluorescent proteins

LOTTI, MARINA;
2012

Abstract

Intrinsically disordered proteins are being paid an increasing amount of interest due to the understanding of the crucial role that flexible regions play in molecular recognition and in signaling. Accordingly, reports focusing on the structural and functional characterization of intrinsically disordered proteins or regions are growing exponentially. Relatively few studies have however been reported on the mutual effects of ordered and disordered moieties in artificial fusion proteins. In this review, we focus on the few available experimental data based on the use of chimeras in which fluorescent proteins were fused to disordered domains of different lengths, compactness and propensity to form secondary structures. The impact of the artificial fusion on the conformational and functional properties of the resulting proteins is discussed.
Articolo in rivista - Articolo scientifico
intrinsically disordered proteins, protein folding, protein solubility
English
2012
8
1
105
113
none
Lotti, M., Longhi, S. (2012). Mutual effects of disorder and order in fusion proteins between intrinsically disordered domains and fluorescent proteins. MOLECULAR BIOSYSTEMS, 8(1), 105-113 [10.1039/c1mb05244f].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/31494
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