During the winter of 2009, we observed an outbreak of severe respiratory disease in Symphysodon discus in a fish farm. The disease began with anorexia, respiratory distress and behavioral disorders and caused the death of all affected juveniles (80%) within a few days. Gill filaments were inflamed, hyperplastic and necrotic, and were infected with Sciadicleithrum variabilum. We amplified the cytochrome oxidase I (COI) gene of the monogenean using PCR and sequenced the gene as a molecular marker to rapidly identify the species. We demonstrated successful sequencing of the COI gene for S. variabilum identification, together with the need to monitor this pathogen in fish farms. © 2012 The Japanese Society of Fish Pathology.
Aquaro, G., Salogni, C., Galli, P., Gibelli, L., Gelmetti, D. (2012). Sciadicleithrum variabilum (Dactylogyridae: Monogenea) Infection in Symphysodon discus: a Case Report. FISH PATHOLOGY, 47(1), 23-26 [10.3147/jsfp.47.23].
Sciadicleithrum variabilum (Dactylogyridae: Monogenea) Infection in Symphysodon discus: a Case Report
AQUARO, GIOVANNI;GALLI, PAOLO;
2012
Abstract
During the winter of 2009, we observed an outbreak of severe respiratory disease in Symphysodon discus in a fish farm. The disease began with anorexia, respiratory distress and behavioral disorders and caused the death of all affected juveniles (80%) within a few days. Gill filaments were inflamed, hyperplastic and necrotic, and were infected with Sciadicleithrum variabilum. We amplified the cytochrome oxidase I (COI) gene of the monogenean using PCR and sequenced the gene as a molecular marker to rapidly identify the species. We demonstrated successful sequencing of the COI gene for S. variabilum identification, together with the need to monitor this pathogen in fish farms. © 2012 The Japanese Society of Fish Pathology.
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