The Museo Nazionale della Scienza e della Tecnologia "Leonardo da Vinci" in Milan is exposing two pairs of canal lock gates, used to control the water flow in Milan canal system, whose design appears in the Leonardo s Codex Atlanticus. The wood present in the gates has been deeply characterised by mean of a multidisciplinary investigation involving i) DNA barcoding of wood fragments; ii) microbial community characterisation, and iii) chemical analyses. DNA barcoding revealed that two fragments of the gates belonged to wood species widely used in the middle age: Fagus sylvatica and Picea abies. The chemical characterisations were based on the use of ionic liquid as dissolving medium in order to analyse the entire cell wall material by means of Gel Permeation Chromatography (GPC) and 2DNMR-HSQC techniques. This multidisciplinary analytical approach was able to highlight the complex nature of the degradation occurred during the gate operation (XVI-XVIII centuries): An intricate interplay between microbial populations (i.e. Shewanella), inorganic factors (i.e. iron from nails), physical factors and the lignocellulosic material.

Zoia, L., Salanti, A., Giorgione, C., Gentili, R., Citterio, S., Gandolfi, I., et al. (2021). Integrated biological and chemical characterisation of a pair of leonardesque canal lock gates. PLOS ONE, 16(3) [10.1371/journal.pone.0247478].

Integrated biological and chemical characterisation of a pair of leonardesque canal lock gates

Zoia L.
;
Salanti A.;Gentili R.;Citterio S.;Gandolfi I.;Franzetti A.;Orlandi M.
2021

Abstract

The Museo Nazionale della Scienza e della Tecnologia "Leonardo da Vinci" in Milan is exposing two pairs of canal lock gates, used to control the water flow in Milan canal system, whose design appears in the Leonardo s Codex Atlanticus. The wood present in the gates has been deeply characterised by mean of a multidisciplinary investigation involving i) DNA barcoding of wood fragments; ii) microbial community characterisation, and iii) chemical analyses. DNA barcoding revealed that two fragments of the gates belonged to wood species widely used in the middle age: Fagus sylvatica and Picea abies. The chemical characterisations were based on the use of ionic liquid as dissolving medium in order to analyse the entire cell wall material by means of Gel Permeation Chromatography (GPC) and 2DNMR-HSQC techniques. This multidisciplinary analytical approach was able to highlight the complex nature of the degradation occurred during the gate operation (XVI-XVIII centuries): An intricate interplay between microbial populations (i.e. Shewanella), inorganic factors (i.e. iron from nails), physical factors and the lignocellulosic material.
Articolo in rivista - Articolo scientifico
archaeological wood, chemical characterisation, DNA barcoding, microbial community
English
10-mar-2021
2021
16
3
e0247478
open
Zoia, L., Salanti, A., Giorgione, C., Gentili, R., Citterio, S., Gandolfi, I., et al. (2021). Integrated biological and chemical characterisation of a pair of leonardesque canal lock gates. PLOS ONE, 16(3) [10.1371/journal.pone.0247478].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/308592
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