Osteobiol influences osteogenic differentiation of adipose derived stem cells

OsteoBiol(R) is a cortical collagenated porcine bone largely employed as substitute for bone grafting. In animal model porcine bone showed good biocompatibility and osteoconductive properties. To study how cortical porcine bone can induce osteoblast differentiation and proliferation in mesenchymal stem cells, the expression levels of bone related genes (RUNX2, SP7, ALPL, SPP1, COL1A1, COL3A1 and FOSL1) and mesenchymal stem cells marker (ENG) were measured in Adipose Derived stem cells (ADSCs) and Human Osteoblasts (HOb) cultivated with OsteoBiol(R). OsteoBiol(R) induces up-regulation of SPP1 and ALPL during the first 15 days of treatment both in treated ADSCs than in treated HOb. In particular the up-regulation of SPP1 demonstrated that this biomaterial is actively resorbed by human osteoclasts