A colorimetric assay, exploiting the combination of loop-mediated isothermal amplification (LAMP) with DNA barcoding, was developed to address the authentication of some cephalopod species, a relevant group in the context of seafood traceability, due to the intensive processing from the fishing sites to the shelf. The discriminating strategy relies on accurate design of species-specific LAMP primers within the conventional 5' end of the mitochondrial COI DNA barcode region and allows for the identification of Loligo vulgaris among two closely related and less valuable species. The assay, coupled to rapid genomic DNA extraction, is suitable for large-scale screenings and on-site applications due to its easy procedures, with fast (30 min) and visual readout.

Tatulli, G., Cecere, P., Maggioni, D., Galimberti, A., Pompa, P. (2020). A Rapid Colorimetric Assay for On-Site Authentication of Cephalopod Species. BIOSENSORS, 10(12) [10.3390/bios10120190].

A Rapid Colorimetric Assay for On-Site Authentication of Cephalopod Species

Maggioni, Davide;Galimberti, Andrea
;
2020

Abstract

A colorimetric assay, exploiting the combination of loop-mediated isothermal amplification (LAMP) with DNA barcoding, was developed to address the authentication of some cephalopod species, a relevant group in the context of seafood traceability, due to the intensive processing from the fishing sites to the shelf. The discriminating strategy relies on accurate design of species-specific LAMP primers within the conventional 5' end of the mitochondrial COI DNA barcode region and allows for the identification of Loligo vulgaris among two closely related and less valuable species. The assay, coupled to rapid genomic DNA extraction, is suitable for large-scale screenings and on-site applications due to its easy procedures, with fast (30 min) and visual readout.
Articolo in rivista - Articolo scientifico
cephalopod species; colorimetric test; food authentication; genetic traceability; LAMP; on-site test;
English
24-nov-2020
2020
10
12
190
open
Tatulli, G., Cecere, P., Maggioni, D., Galimberti, A., Pompa, P. (2020). A Rapid Colorimetric Assay for On-Site Authentication of Cephalopod Species. BIOSENSORS, 10(12) [10.3390/bios10120190].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/296422
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