L-glutamate (Glu) is the major excitatory transmitter in mammalian brain. Inadequate concentration of Glu in the brain correlates to mood disorder. In industry, Glu is used as a flavour enhancer in food and in foodstuff processing. A high concentration of Glu has several effects on human health such as hypersensitive effects, headache and stomach pain. The presence of Glu in food can be detected by different analytical methods based on chromatography, or capillary electrophoresis or amperometric techniques. We have isolated and characterized a glutamate-binding protein (GluB) from the Gram-positive bacteria Corynebacterium glutamicum. Together with GluC protein, GluD protein and the cytoplasmic protein GluA, GluB permits the transport of Glu in/out of cell. In this study, we have investigated the binding features of GluB as well as the effect of temperature on its structure both in the absence and in the presence of Glu. The results have showed that GluB has a high affinity and selectivity versus Glu (nanomolar range) and the presence of the ligand induces a higher thermal stability of the protein structure.

Capo, A., Natalello, A., Marienhagen, J., Pennacchio, A., Camarca, A., Di Giovanni, S., et al. (2020). Structural features of the glutamate-binding protein from Corynebacterium glutamicum. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 162, 903-912 [10.1016/j.ijbiomac.2020.06.197].

Structural features of the glutamate-binding protein from Corynebacterium glutamicum

Natalello A.
Secondo
Membro del Collaboration Group
;
2020

Abstract

L-glutamate (Glu) is the major excitatory transmitter in mammalian brain. Inadequate concentration of Glu in the brain correlates to mood disorder. In industry, Glu is used as a flavour enhancer in food and in foodstuff processing. A high concentration of Glu has several effects on human health such as hypersensitive effects, headache and stomach pain. The presence of Glu in food can be detected by different analytical methods based on chromatography, or capillary electrophoresis or amperometric techniques. We have isolated and characterized a glutamate-binding protein (GluB) from the Gram-positive bacteria Corynebacterium glutamicum. Together with GluC protein, GluD protein and the cytoplasmic protein GluA, GluB permits the transport of Glu in/out of cell. In this study, we have investigated the binding features of GluB as well as the effect of temperature on its structure both in the absence and in the presence of Glu. The results have showed that GluB has a high affinity and selectivity versus Glu (nanomolar range) and the presence of the ligand induces a higher thermal stability of the protein structure.
Articolo in rivista - Articolo scientifico
Corynebacterium glutamicum; Fluorescence spectroscopy; FT-IR; Glutamate binding protein
English
25-giu-2020
2020
162
903
912
none
Capo, A., Natalello, A., Marienhagen, J., Pennacchio, A., Camarca, A., Di Giovanni, S., et al. (2020). Structural features of the glutamate-binding protein from Corynebacterium glutamicum. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 162, 903-912 [10.1016/j.ijbiomac.2020.06.197].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/294960
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