The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. After incubation of Pseudomonas cultures with 1 mM of AgNO3 at 22 °C, we obtained AgNPs within 24 h. Scanning electron (SEM) and transmission electron microscopy (TEM) revealed spherical polydispersed AgNPs in the size range of 20-70 nm. The average size was approximately 50 nm. Energy dispersive X-ray spectroscopy (EDS) showed the presence of a high intensity absorption peak at 3 keV, a distinctive property of nanocrystalline silver products. Fourier transform infrared (FTIR) spectroscopy found the presence of a high amount of AgNP-stabilizing proteins and other secondary metabolites. X-ray diffraction (XRD) revealed a face-centred cubic (fcc) diffraction spectrum with a crystalline nature. A comparative study between the chemically synthesized and Pseudomonas AgNPs revealed a higher antibacterial activity of the latter against common nosocomial pathogen microorganisms, including Escherichia coli, Staphylococcus aureus and Candida albicans. This study reports an efficient, rapid synthesis of stable AgNPs by a new Pseudomonas strain with high antimicrobial activity.
John, M., Nagoth, J., Ramasamy, K., Mancini, A., Giuli, G., Natalello, A., et al. (2020). Synthesis of bioactive silver nanoparticles by a pseudomonas strain associated with the antarctic psychrophilic protozoon euplotes focardii. MARINE DRUGS, 18(1).
Citazione: | John, M., Nagoth, J., Ramasamy, K., Mancini, A., Giuli, G., Natalello, A., et al. (2020). Synthesis of bioactive silver nanoparticles by a pseudomonas strain associated with the antarctic psychrophilic protozoon euplotes focardii. MARINE DRUGS, 18(1). | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | Synthesis of bioactive silver nanoparticles by a pseudomonas strain associated with the antarctic psychrophilic protozoon euplotes focardii | |
Autori: | John, M; Nagoth, J; Ramasamy, K; Mancini, A; Giuli, G; Natalello, A; Ballarini, P; Miceli, C; Pucciarelli, S | |
Autori: | ||
Data di pubblicazione: | 2020 | |
Lingua: | English | |
Rivista: | MARINE DRUGS | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.3390/md18010038 | |
Appare nelle tipologie: | 01 - Articolo su rivista |