The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.

Sgambato, A., Pastori, V., Russo, L., Vesentini, S., Lecchi, M., Cipolla, L. (2020). Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines. MOLECULES, 25(19) [10.3390/molecules25194361].

Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

Sgambato A.
;
Pastori V.
;
Russo L.
;
Lecchi M.
;
Cipolla L.
2020

Abstract

The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.
Articolo in rivista - Articolo scientifico
Biomaterials; Carbohydrates; Chondroitin sulfate; Collagen; F-11 cell line; Galactose; Glucose; Sialic acid
English
23-set-2020
2020
25
19
4361
none
Sgambato, A., Pastori, V., Russo, L., Vesentini, S., Lecchi, M., Cipolla, L. (2020). Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines. MOLECULES, 25(19) [10.3390/molecules25194361].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/289471
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