Key stereoelectronic properties of Desulfovibrio desulfuricans [FeFe]-hydrogenase (DdH) were investigated by quantum mechanical description of its complete inorganic core, which includes a Fe6S6 active site (the H-cluster), as well as two ancillary Fe4S4 assemblies (the F and F' clusters). The partially oxidized, active-ready form of DdH is able to efficiently bind dihydrogen, thus starting H2 oxidation catalysis. The calculations allow us to unambiguously assign a mixed Fe(II)Fe(I) state to the catalytic core of the activeready enzyme and show that H2 uptake exerts subtle, yet crucial influences on the redox properties of DdH. In fact, H2 binding can promote electron transfer from the H-cluster to the solvent-exposed F'-cluster, thanks to a 50% decrease of the energy gap between the HOMO (that is localized on the H-cluster) and the LUMO (which is centered on the F'-cluster). Our results also indicate that the binding of the redox partners of DdH in proximity of its F'-cluster can trigger one-electron oxidation of the H2-bound enzyme, a process that is expected to have an important role in H2 activation. Our findings are analyzed not only from a mechanistic perspective, but also in consideration of the physiological role of DdH. In fact, this enzyme is known to be able to catalyze both the oxidation and the evolution of H2, depending on the cellular metabolic requirements. Hints for the design of targeted mutations that could lead to the enhancement of the oxidizing properties of DdH are proposed and discussed. © 2011 American Chemical Society.

Greco, C., Bruschi, M., Fantucci, P., Ryde, U., DE GIOIA, L. (2011). Mechanistic and Physiological Implications of the Interplay among Iron-Sulfur Clusters in [FeFe]-Hydrogenases. A QM/MM Perspective. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(46), 18742-18749 [10.1021/ja205542k].

Mechanistic and Physiological Implications of the Interplay among Iron-Sulfur Clusters in [FeFe]-Hydrogenases. A QM/MM Perspective

GRECO, CLAUDIO;BRUSCHI, MAURIZIO;FANTUCCI, PIERCARLO;DE GIOIA, LUCA
2011

Abstract

Key stereoelectronic properties of Desulfovibrio desulfuricans [FeFe]-hydrogenase (DdH) were investigated by quantum mechanical description of its complete inorganic core, which includes a Fe6S6 active site (the H-cluster), as well as two ancillary Fe4S4 assemblies (the F and F' clusters). The partially oxidized, active-ready form of DdH is able to efficiently bind dihydrogen, thus starting H2 oxidation catalysis. The calculations allow us to unambiguously assign a mixed Fe(II)Fe(I) state to the catalytic core of the activeready enzyme and show that H2 uptake exerts subtle, yet crucial influences on the redox properties of DdH. In fact, H2 binding can promote electron transfer from the H-cluster to the solvent-exposed F'-cluster, thanks to a 50% decrease of the energy gap between the HOMO (that is localized on the H-cluster) and the LUMO (which is centered on the F'-cluster). Our results also indicate that the binding of the redox partners of DdH in proximity of its F'-cluster can trigger one-electron oxidation of the H2-bound enzyme, a process that is expected to have an important role in H2 activation. Our findings are analyzed not only from a mechanistic perspective, but also in consideration of the physiological role of DdH. In fact, this enzyme is known to be able to catalyze both the oxidation and the evolution of H2, depending on the cellular metabolic requirements. Hints for the design of targeted mutations that could lead to the enhancement of the oxidizing properties of DdH are proposed and discussed. © 2011 American Chemical Society.
Articolo in rivista - Articolo scientifico
FE-ONLY HYDROGENASE; DESULFOVIBRIO-VULGARIS HILDENBOROUGH; DENSITY-FUNCTIONAL CALCULATIONS; ACTIVE-SITE; H-CLUSTER; CLOSTRIDIUM-PASTEURIANUM; INFRARED-SPECTROSCOPY; ELECTRONIC-STRUCTURE; LIGHT SENSITIVITY; REDOX PROPERTIES
English
2011
133
46
18742
18749
none
Greco, C., Bruschi, M., Fantucci, P., Ryde, U., DE GIOIA, L. (2011). Mechanistic and Physiological Implications of the Interplay among Iron-Sulfur Clusters in [FeFe]-Hydrogenases. A QM/MM Perspective. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(46), 18742-18749 [10.1021/ja205542k].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/28603
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