The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.

Pedranzini, L., Mottadelli, F., Ronzoni, S., Rossella, F., Ferracin, M., Magnani, I., et al. (2010). Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment. LEUKEMIA RESEARCH, 34(10), 1287-1295 [10.1016/j.leukres.2010.02.012].

Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment

ROVERSI, GAIA;
2010

Abstract

The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.
Articolo in rivista - Articolo scientifico
Comparative Genomic Hybridization; Cell Line, Tumor; Gene Expression Profiling; Proto-Oncogene Proteins c-kit; Chromosomes, Human, Pair 4; MicroRNAs; Antigens, CD34; Leukemia, Myeloid, Acute; Immunophenotyping; Antigens, CD38; Humans
English
2010
34
10
1287
1295
none
Pedranzini, L., Mottadelli, F., Ronzoni, S., Rossella, F., Ferracin, M., Magnani, I., et al. (2010). Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment. LEUKEMIA RESEARCH, 34(10), 1287-1295 [10.1016/j.leukres.2010.02.012].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/28429
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