The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.

Pedranzini, L., Mottadelli, F., Ronzoni, S., Rossella, F., Ferracin, M., Magnani, I., et al. (2010). Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment. LEUKEMIA RESEARCH, 34(10), 1287-1295 [10.1016/j.leukres.2010.02.012].

Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment

ROVERSI, GAIA;
2010

Abstract

The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.
Articolo in rivista - Articolo scientifico
Comparative Genomic Hybridization; Cell Line, Tumor; Gene Expression Profiling; Proto-Oncogene Proteins c-kit; Chromosomes, Human, Pair 4; MicroRNAs; Antigens, CD34; Leukemia, Myeloid, Acute; Immunophenotyping; Antigens, CD38; Humans
English
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1295
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Pedranzini, L., Mottadelli, F., Ronzoni, S., Rossella, F., Ferracin, M., Magnani, I., et al. (2010). Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment. LEUKEMIA RESEARCH, 34(10), 1287-1295 [10.1016/j.leukres.2010.02.012].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/28429
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