One of the major drawbacks that limits the clinical application of nanoparticles is the lack of preliminary investigations related to their biocompatibility, biodegradability and biodistribution. In this work, biodegradable PEGylated polymer nanoparticles (NPs) have been synthesized by using macromonomers based on poly(ε-caprolaconte) oligomers. More in detail, NPs have been produced by adopting a surfactant-free semibatch emulsion polymerization process using PEG chains as a stabilizing agent. The NPs were also labeled with rhodamine B covalently bound to the NPs to quantitatively study their biodistribution in vivo. NPs were investigated in both in vitro and in vivo preclinical systems to study their biodistribution in mice bearing B16/F10 melanoma, as well as their biocompatibility and biodegradability. The NP concentration was evaluated in different tissues at several times after intravenous injection. The disappearance of the NPs from the plasma was biphasic, with distribution and elimination half-lives of 30 min and 15 h, respectively. NPs were retained in tumors and in filter organs for a long time, were still detectable after 7 d and maintained a steady concentration in the tumor for 120 h. 48 h after injection, 70± 15% of the inoculated NPs were excreted in the feces. The favorable tumor uptake, fast excretion and absence of cytotoxicity foster the further development of produced NPs as drug delivery carriers. © 2014 IOP Publishing Ltd.

Lupi, M., Colombo, C., Frapolli, R., Ferrari, R., Sitia, L., Dragoni, L., et al. (2014). A biodistribution study of PEGylated PCL-based nanoparticles in C57BL/6 mice bearing B16/F10 melanoma. NANOTECHNOLOGY, 25(33) [10.1088/0957-4484/25/33/335706].

A biodistribution study of PEGylated PCL-based nanoparticles in C57BL/6 mice bearing B16/F10 melanoma

Licandro S. A.;
2014

Abstract

One of the major drawbacks that limits the clinical application of nanoparticles is the lack of preliminary investigations related to their biocompatibility, biodegradability and biodistribution. In this work, biodegradable PEGylated polymer nanoparticles (NPs) have been synthesized by using macromonomers based on poly(ε-caprolaconte) oligomers. More in detail, NPs have been produced by adopting a surfactant-free semibatch emulsion polymerization process using PEG chains as a stabilizing agent. The NPs were also labeled with rhodamine B covalently bound to the NPs to quantitatively study their biodistribution in vivo. NPs were investigated in both in vitro and in vivo preclinical systems to study their biodistribution in mice bearing B16/F10 melanoma, as well as their biocompatibility and biodegradability. The NP concentration was evaluated in different tissues at several times after intravenous injection. The disappearance of the NPs from the plasma was biphasic, with distribution and elimination half-lives of 30 min and 15 h, respectively. NPs were retained in tumors and in filter organs for a long time, were still detectable after 7 d and maintained a steady concentration in the tumor for 120 h. 48 h after injection, 70± 15% of the inoculated NPs were excreted in the feces. The favorable tumor uptake, fast excretion and absence of cytotoxicity foster the further development of produced NPs as drug delivery carriers. © 2014 IOP Publishing Ltd.
Articolo in rivista - Articolo scientifico
B16/F10 melanoma, biodistribution, emulsion polymerization, nanoparticles, poly ethylene glyco, poly-ε-caprolactone, Animals, Cell Line, Tumor, Disease Models, Animal, Dose-Response Relationship, Drug, Female, Melanoma, Experimental, Mice, Mice, Inbred C57BL, Nanoparticles, Particle Size, Polyethylene Glycols, Polymers, Rhodamines, Tissue Distribution, Drug Delivery Systems
English
2014
25
33
335706
none
Lupi, M., Colombo, C., Frapolli, R., Ferrari, R., Sitia, L., Dragoni, L., et al. (2014). A biodistribution study of PEGylated PCL-based nanoparticles in C57BL/6 mice bearing B16/F10 melanoma. NANOTECHNOLOGY, 25(33) [10.1088/0957-4484/25/33/335706].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/280750
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