Background: Trabectedin is an anticancer agent registered for the second-line treatment of soft tissue sarcoma and ovarian cancer. No preclinical data are available on its tumor distribution, so a method for quantification in neoplastic tissues is required. Methods/results: We validated an LC-MS/MS assay determining the recovery, sensitivity, linearity, precision and accuracy in mouse tumor and liver samples. The limit of quantification was 0.10 ng/ml with a curve range of 0.10-3.00 ng/ml (accuracy 96.1-102.1%). Inter-day precision and accuracy of QCs were 6.0-8.2 and 97.0-102.6% respectively. The method was applied in mesothelioma xenografts treated with therapeutic doses. Conclusion: The method was validated for measuring trabectedin in tissues. In a mesothelioma xenograft model, trabectedin distributed preferentially in tumor compared with liver.

Ceriani, L., Ferrari, M., Zangarini, M., Licandro, S., Bello, E., Frapolli, R., et al. (2015). HPLC-MS/MS method to measure trabectedin in tumors: Preliminary PK study in a mesothelioma xenograft model. BIOANALYSIS, 7(15), 1831-1842 [10.4155/bio.15.117].

HPLC-MS/MS method to measure trabectedin in tumors: Preliminary PK study in a mesothelioma xenograft model

Licandro S. A.;
2015

Abstract

Background: Trabectedin is an anticancer agent registered for the second-line treatment of soft tissue sarcoma and ovarian cancer. No preclinical data are available on its tumor distribution, so a method for quantification in neoplastic tissues is required. Methods/results: We validated an LC-MS/MS assay determining the recovery, sensitivity, linearity, precision and accuracy in mouse tumor and liver samples. The limit of quantification was 0.10 ng/ml with a curve range of 0.10-3.00 ng/ml (accuracy 96.1-102.1%). Inter-day precision and accuracy of QCs were 6.0-8.2 and 97.0-102.6% respectively. The method was applied in mesothelioma xenografts treated with therapeutic doses. Conclusion: The method was validated for measuring trabectedin in tissues. In a mesothelioma xenograft model, trabectedin distributed preferentially in tumor compared with liver.
Articolo in rivista - Articolo scientifico
Animals, Antineoplastic Agents, Alkylating, Chromatography, High Pressure Liquid, Dioxoles, Female, Humans, Mesothelioma, Mice, Nude, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Tetrahydroisoquinolines, Trabectedin, Xenograft Model Antitumor Assays
English
2015
7
15
1831
1842
none
Ceriani, L., Ferrari, M., Zangarini, M., Licandro, S., Bello, E., Frapolli, R., et al. (2015). HPLC-MS/MS method to measure trabectedin in tumors: Preliminary PK study in a mesothelioma xenograft model. BIOANALYSIS, 7(15), 1831-1842 [10.4155/bio.15.117].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/280746
Citazioni
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
Social impact