Chimeric Antigen Receptor (CAR) T-cell therapy has become a new therapeutic reality for refractory and relapsed leukemia patients and is also emerging as a potential therapeutic option in solid tumors. Viral vector-based CAR T-cells initially drove these successful efforts; however, high costs and cumbersome manufacturing processes have limited the widespread clinical implementation of CAR T-cell therapy. Here we will discuss the state of the art of the transposon-based gene transfer and its application in CAR T immunotherapy, specifically focusing on the Sleeping Beauty (SB) transposon system, as a valid cost-effective and safe option as compared to the viral vector-based systems. A general overview of SB transposon system applications will be provided, with an update of major developments, current clinical trials achievements and future perspectives exploiting SB for CAR T-cell engineering. After the first clinical successes achieved in the context of B-cell neoplasms, we are now facing a new era and it is paramount to advance gene transfer technology to fully exploit the potential of CAR T-cells towards next-generation immunotherapy.
Magnani, C., Tettamanti, S., Alberti, G., Pisani, I., Biondi, A., Serafini, M., et al. (2020). Transposon-Based CAR T Cells in Acute Leukemias: Where are We Going?. CELLS, 9(6), 1337 [10.3390/cells9061337].
|Citazione:||Magnani, C., Tettamanti, S., Alberti, G., Pisani, I., Biondi, A., Serafini, M., et al. (2020). Transposon-Based CAR T Cells in Acute Leukemias: Where are We Going?. CELLS, 9(6), 1337 [10.3390/cells9061337].|
|Tipo:||Articolo in rivista - Review Essay|
|Carattere della pubblicazione:||Scientifica|
|Presenza di un coautore afferente ad Istituzioni straniere:||No|
|Titolo:||Transposon-Based CAR T Cells in Acute Leukemias: Where are We Going?|
|Autori:||Magnani, C; Tettamanti, S; Alberti, G; Pisani, I; Biondi, A; Serafini, M; Gaipa, G|
BIONDI, ANDREA (Corresponding)
|Data di pubblicazione:||2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/cells9061337|
|Appare nelle tipologie:||01 - Articolo su rivista|