Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. In this respect, azobenzene-based photoswitches are promising nanoscale tools for neuronal photostimulation. Here we engineered a novel light-sensitive azobenzene compound (Ziapin2) that stably partitions into the plasma membrane, and causes its thinning through trans-dimerization in the dark, resulting in an increased membrane capacitance a steady state. We demonstrated that in neurons loaded with the compound, millisecond pulses of visible light induce a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. These effects are persistent and can be evoked in vivo up to 7 days, proving the potential of Ziapin2 for the modulation of membrane capacitance in the millisecond time scale, without directly affecting ion channels or local temperature.

Difrancesco, M., Lodola, F., Colombo, E., Maragliano, L., Bramini, M., Paternò, G., et al. (2020). Neuronal firing modulation by a membrane-targeted photoswitch. NATURE NANOTECHNOLOGY, 15(4), 296-306 [10.1038/s41565-019-0632-6].

Neuronal firing modulation by a membrane-targeted photoswitch

Lodola F
Co-primo
;
2020

Abstract

Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. In this respect, azobenzene-based photoswitches are promising nanoscale tools for neuronal photostimulation. Here we engineered a novel light-sensitive azobenzene compound (Ziapin2) that stably partitions into the plasma membrane, and causes its thinning through trans-dimerization in the dark, resulting in an increased membrane capacitance a steady state. We demonstrated that in neurons loaded with the compound, millisecond pulses of visible light induce a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. These effects are persistent and can be evoked in vivo up to 7 days, proving the potential of Ziapin2 for the modulation of membrane capacitance in the millisecond time scale, without directly affecting ion channels or local temperature.
Articolo in rivista - Articolo scientifico
Nanotechnology, Physiology, Neurobiology, light-stimulation
English
296
306
11
Difrancesco, M., Lodola, F., Colombo, E., Maragliano, L., Bramini, M., Paternò, G., et al. (2020). Neuronal firing modulation by a membrane-targeted photoswitch. NATURE NANOTECHNOLOGY, 15(4), 296-306 [10.1038/s41565-019-0632-6].
File in questo prodotto:
File Dimensione Formato  
2020_DiFrancesco_Lodola et al.pdf

Solo gestori archivio

Dimensione 5.86 MB
Formato Adobe PDF
5.86 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/276335
Citazioni
  • Scopus 41
  • ???jsp.display-item.citation.isi??? 34
Social impact