The human carcinogenicity evaluation of chemicals has a great impact on public health. In vitro methods, such as the cell transformation assay (CTA), allow for a fast and reliable assessment of the carcinogenic potential of a chemical compound in comparison with the standard two-year bioassay. The scoring and classification of foci in selected cell lines is performed, after staining, by light microscopy. Foci can be separated into three classes: type I, which are scored as non-transformed, and types II and III that are considered to include fully transformed foci. However, in a number of cases, even an expert is uncertain about the attribution of a focus to a given class, due to its mixed or intermediate nature. Here, we suggest a simple approach to classifying mixed or intermediate foci by exploiting the quantitative information available from images, which is captured by statistical descriptors. A quantitative index is proposed, to describe the degree of dissimilarity of mixed and intermediate images to the three well-distinguished classes

Procaccianti, C., Stefanini, F., & Urani, C. (2011). The cell trasformation assay: Toward a statistical classification of mixed and intermediate foci images. ATLA. ALTERNATIVES TO LABORATORY ANIMALS, 39(1), 23-36 [10.1177/026119291103900118].

The cell trasformation assay: Toward a statistical classification of mixed and intermediate foci images

Procaccianti, C;Urani, C.
2011

Abstract

The human carcinogenicity evaluation of chemicals has a great impact on public health. In vitro methods, such as the cell transformation assay (CTA), allow for a fast and reliable assessment of the carcinogenic potential of a chemical compound in comparison with the standard two-year bioassay. The scoring and classification of foci in selected cell lines is performed, after staining, by light microscopy. Foci can be separated into three classes: type I, which are scored as non-transformed, and types II and III that are considered to include fully transformed foci. However, in a number of cases, even an expert is uncertain about the attribution of a focus to a given class, due to its mixed or intermediate nature. Here, we suggest a simple approach to classifying mixed or intermediate foci by exploiting the quantitative information available from images, which is captured by statistical descriptors. A quantitative index is proposed, to describe the degree of dissimilarity of mixed and intermediate images to the three well-distinguished classes
No
Articolo in rivista - Articolo scientifico
Scientifica
chemical carcinogenesis; in vitro cell trasformation; cluster analysis; quantitative morphology
English
23
36
14
Procaccianti, C., Stefanini, F., & Urani, C. (2011). The cell trasformation assay: Toward a statistical classification of mixed and intermediate foci images. ATLA. ALTERNATIVES TO LABORATORY ANIMALS, 39(1), 23-36 [10.1177/026119291103900118].
Procaccianti, C; Stefanini, F; Urani, C
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/26506
Citazioni
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 4
Social impact