The human carcinogenicity evaluation of chemicals has a great impact on public health. In vitro methods, such as the cell transformation assay (CTA), allow for a fast and reliable assessment of the carcinogenic potential of a chemical compound in comparison with the standard two-year bioassay. The scoring and classification of foci in selected cell lines is performed, after staining, by light microscopy. Foci can be separated into three classes: type I, which are scored as non-transformed, and types II and III that are considered to include fully transformed foci. However, in a number of cases, even an expert is uncertain about the attribution of a focus to a given class, due to its mixed or intermediate nature. Here, we suggest a simple approach to classifying mixed or intermediate foci by exploiting the quantitative information available from images, which is captured by statistical descriptors. A quantitative index is proposed, to describe the degree of dissimilarity of mixed and intermediate images to the three well-distinguished classes

Procaccianti, C., Stefanini, F., Urani, C. (2011). The cell trasformation assay: Toward a statistical classification of mixed and intermediate foci images. ATLA. ALTERNATIVES TO LABORATORY ANIMALS, 39(1), 23-36 [10.1177/026119291103900118].

The cell trasformation assay: Toward a statistical classification of mixed and intermediate foci images

Procaccianti, C;Urani, C.
2011

Abstract

The human carcinogenicity evaluation of chemicals has a great impact on public health. In vitro methods, such as the cell transformation assay (CTA), allow for a fast and reliable assessment of the carcinogenic potential of a chemical compound in comparison with the standard two-year bioassay. The scoring and classification of foci in selected cell lines is performed, after staining, by light microscopy. Foci can be separated into three classes: type I, which are scored as non-transformed, and types II and III that are considered to include fully transformed foci. However, in a number of cases, even an expert is uncertain about the attribution of a focus to a given class, due to its mixed or intermediate nature. Here, we suggest a simple approach to classifying mixed or intermediate foci by exploiting the quantitative information available from images, which is captured by statistical descriptors. A quantitative index is proposed, to describe the degree of dissimilarity of mixed and intermediate images to the three well-distinguished classes
Articolo in rivista - Articolo scientifico
chemical carcinogenesis; in vitro cell trasformation; cluster analysis; quantitative morphology
English
2011
39
1
23
36
none
Procaccianti, C., Stefanini, F., Urani, C. (2011). The cell trasformation assay: Toward a statistical classification of mixed and intermediate foci images. ATLA. ALTERNATIVES TO LABORATORY ANIMALS, 39(1), 23-36 [10.1177/026119291103900118].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/26506
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