Out of 130 ovarian cancer patients the DNA index of cells from ovarian carcinoma was studied in 56 cases in which cytospin preparations showed the presence of atypical cells. In 24 patients the population had a diploid DNA index (1.0) and in the others the DNA index ranged from 1.2 to 2.0 (tetraploid). No hypodiploid or hypertetraploid populations were detected. Repeated samples from the same patients did not show any significant differences and primary culture did not alter the DNA index. In contrast, cell cycle phase distribution differed greatly from sample to sample, as also the ratio between DNA diploid and DNA aneuploid populations. Primary culture was successful in 57% of the tumours, with a higher percentage of success in DNA aneuploid tumours. After primary culture the ratio between DNA aneuploid cells and DNA diploid cells increased. In relation to the histological gradings of malignancy, DNA aneuploid cells clustered in the highest grade of malignancy. The mean S-phase for tumours with a DNA index of 1.0 was 3.5 and 14.1% for those with DNA index >1. Ovarian carcinomas show a large difference in DNA index between patients even after primary culture. © The Macmillan Press Ltd., 1985

Erba, E., Vaghi, M., Pepe, S., Amato, G., Bistolfi, M., Ubeziol, P., et al. (1985). DNA index of ovarian carcinomas from 56 patients: In vivo in vitro studies. BRITISH JOURNAL OF CANCER, 52(4), 565-573 [10.1038/bjc.1985.228].

DNA index of ovarian carcinomas from 56 patients: In vivo in vitro studies

Mangioni C.;Landoni F.;
1985

Abstract

Out of 130 ovarian cancer patients the DNA index of cells from ovarian carcinoma was studied in 56 cases in which cytospin preparations showed the presence of atypical cells. In 24 patients the population had a diploid DNA index (1.0) and in the others the DNA index ranged from 1.2 to 2.0 (tetraploid). No hypodiploid or hypertetraploid populations were detected. Repeated samples from the same patients did not show any significant differences and primary culture did not alter the DNA index. In contrast, cell cycle phase distribution differed greatly from sample to sample, as also the ratio between DNA diploid and DNA aneuploid populations. Primary culture was successful in 57% of the tumours, with a higher percentage of success in DNA aneuploid tumours. After primary culture the ratio between DNA aneuploid cells and DNA diploid cells increased. In relation to the histological gradings of malignancy, DNA aneuploid cells clustered in the highest grade of malignancy. The mean S-phase for tumours with a DNA index of 1.0 was 3.5 and 14.1% for those with DNA index >1. Ovarian carcinomas show a large difference in DNA index between patients even after primary culture. © The Macmillan Press Ltd., 1985
Articolo in rivista - Articolo scientifico
DNA, Neoplasm; Female; Flow Cytometry; Humans; In Vitro Techniques; Interphase; Mitosis; Ovarian Neoplasms; Ploidies; Time Factors
English
565
573
9
Erba, E., Vaghi, M., Pepe, S., Amato, G., Bistolfi, M., Ubeziol, P., et al. (1985). DNA index of ovarian carcinomas from 56 patients: In vivo in vitro studies. BRITISH JOURNAL OF CANCER, 52(4), 565-573 [10.1038/bjc.1985.228].
Erba, E; Vaghi, M; Pepe, S; Amato, G; Bistolfi, M; Ubeziol, P; Mangioni, C; Landoni, F; Morasca, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/264999
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