Multiple myeloma (MM) belongs to hematological cancers and its incidence is increasing worldwide. Despite recent advances in its therapy, MM still causes many deaths every year. In fact, current therapies sometimes fail and are associated with severe adverse effects, including neurotoxicity. As a part of our ongoing efforts to discover new potential therapies against MM, we prepared Hibiscus sabdariffa extracts obtained by a microwave-assisted solvent extraction and investigate their activity by in vitro assays on the RPMI-8226 cell line. The bioguided fractionation of the crude ethanolic extract allowed the identification of HsFC as the most effective extract. We assessed cell viability (MTT and Tripan blue test), cell migration (Boyden chamber assay), and neurotoxicity (DRG neurotoxicity assay). The promising results prompted us to further fractionate HsFC and we obtained two molecules effective against RPMI-8226 cells without neurotoxic effects at their active concentrations. Moreover, both compounds are able to significantly reduce cell migration.

Malacrida, A., Cavalloro, V., Martino, E., Cassetti, A., Nicolini, G., Rigolio, R., et al. (2019). Anti-multiple myeloma potential of secondary metabolites from hibiscus sabdariffa. MOLECULES, 24(13) [10.3390/molecules24132500].

Anti-multiple myeloma potential of secondary metabolites from hibiscus sabdariffa

Malacrida A.;Cassetti A.;Nicolini G.;Rigolio R.;Cavaletti G.;Di Giacomo M.;Miloso M.
2019

Abstract

Multiple myeloma (MM) belongs to hematological cancers and its incidence is increasing worldwide. Despite recent advances in its therapy, MM still causes many deaths every year. In fact, current therapies sometimes fail and are associated with severe adverse effects, including neurotoxicity. As a part of our ongoing efforts to discover new potential therapies against MM, we prepared Hibiscus sabdariffa extracts obtained by a microwave-assisted solvent extraction and investigate their activity by in vitro assays on the RPMI-8226 cell line. The bioguided fractionation of the crude ethanolic extract allowed the identification of HsFC as the most effective extract. We assessed cell viability (MTT and Tripan blue test), cell migration (Boyden chamber assay), and neurotoxicity (DRG neurotoxicity assay). The promising results prompted us to further fractionate HsFC and we obtained two molecules effective against RPMI-8226 cells without neurotoxic effects at their active concentrations. Moreover, both compounds are able to significantly reduce cell migration.
No
Articolo in rivista - Articolo scientifico
Scientifica
Bioguided assay fractionation; Hib-carbaldehyde; Hib-ester; Hibiscus sabdariffa; Multiple myeloma; Nature-aided drug discovery;
Bioguided assay fractionation; Hib-carbaldehyde; Hib-ester; Hibiscus sabdariffa; Multiple myeloma; Nature-aided drug discovery; Antineoplastic Agents, Phytogenic; Cell Line, Tumor; Chemical Fractionation; Chromatography, High Pressure Liquid; Hibiscus; Humans; Mass Spectrometry; Molecular Structure; Multiple Myeloma; Plant Extracts; Secondary Metabolism; Spectrometry, Mass, Electrospray Ionization
English
Malacrida, A., Cavalloro, V., Martino, E., Cassetti, A., Nicolini, G., Rigolio, R., et al. (2019). Anti-multiple myeloma potential of secondary metabolites from hibiscus sabdariffa. MOLECULES, 24(13) [10.3390/molecules24132500].
Malacrida, A; Cavalloro, V; Martino, E; Cassetti, A; Nicolini, G; Rigolio, R; Cavaletti, G; Mannucci, B; Vasile, F; Di Giacomo, M; Collina, S; Miloso, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/261609
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