Mice were immunised with 30S subunits from E. coli and their spleen cells were fused with myeloma cells. From this fusion two monoclonal antibodies were obtained, one of which was shown to be specific for ribosomal protein S3, the other for ribosomal protein S7. The two monoclonal antibodies formed stable complexes with intact 30S subunits and were therefore used for the three-dimensional localisation of ribosomal proteins S3 and S7 on the surface of the E. coli small subunit by immuno electron microscopy. The antibody binding sites determined with the two monoclonal antibodies were found to lie in the same area as those obtained with conventional antibodies. Both proteins S3 and S7 are located on the head of the 30S subunit, close to the one-third/two-thirds partition. Protein S3 is located just above the small lobe, whereas protein S7 is located on the side of the large lobe. © 1984 Springer-Verlag.

Breitenreuter, G., Lotti, M., Stoffler-Meilicke, M., Stoffler, G. (1984). Comparative electron microscopic study on the location of ribosomal proteins S3 and S7 on the surface of the E. coli 30S subunit using monoclonal and conventional antibody. MOLECULAR AND GENERAL GENETICS, 197(2), 189-195 [10.1007/BF00330962].

Comparative electron microscopic study on the location of ribosomal proteins S3 and S7 on the surface of the E. coli 30S subunit using monoclonal and conventional antibody

Lotti, M;
1984

Abstract

Mice were immunised with 30S subunits from E. coli and their spleen cells were fused with myeloma cells. From this fusion two monoclonal antibodies were obtained, one of which was shown to be specific for ribosomal protein S3, the other for ribosomal protein S7. The two monoclonal antibodies formed stable complexes with intact 30S subunits and were therefore used for the three-dimensional localisation of ribosomal proteins S3 and S7 on the surface of the E. coli small subunit by immuno electron microscopy. The antibody binding sites determined with the two monoclonal antibodies were found to lie in the same area as those obtained with conventional antibodies. Both proteins S3 and S7 are located on the head of the 30S subunit, close to the one-third/two-thirds partition. Protein S3 is located just above the small lobe, whereas protein S7 is located on the side of the large lobe. © 1984 Springer-Verlag.
Articolo in rivista - Articolo scientifico
Antibodies, Bacterial; Antibodies, Monoclonal; Bacterial Proteins; Escherichia coli; Microscopy, Electron; Ribosomal Proteins; Ribosomes
English
1984
197
2
189
195
none
Breitenreuter, G., Lotti, M., Stoffler-Meilicke, M., Stoffler, G. (1984). Comparative electron microscopic study on the location of ribosomal proteins S3 and S7 on the surface of the E. coli 30S subunit using monoclonal and conventional antibody. MOLECULAR AND GENERAL GENETICS, 197(2), 189-195 [10.1007/BF00330962].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/260243
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