Lipases (Lip) hydrolyze triglycerides into fatty acids and glycerol. Lip produced by the yeast Candida cylindracea are encoded by multiple genomic sequences. We report the molecular cloning and characterization of three genes from this family. They encode putative mature 57-kDa proteins of 534 amino acids (aa). To date, five Lip-encoding genomic sequences from C. cylindracea have been characterized in our laboratory. The five deduced aa sequences share an overall homology of 80%. These sequences have been aligned with each other and with those of homologous enzymes, the Lip from the mould Geotrichum candidum and the acetylcholinesterase from Torpedo californica, whose three-dimensional structures have been solved by X-ray analysis. The C. cylindracea Lip appear to have a structural organization similar to that described for both enzymes. © 1993.

Lotti, M., Grandori, R., Fusetti, F., Longhi, S., Brocca, S., Tramontane, A., et al. (1993). Cloning and analysis of Candida cylindracea lipase sequences. GENE, 124(1), 45-55 [10.1016/0378-1119(93)90760-Z].

Cloning and analysis of Candida cylindracea lipase sequences

Lotti M.;Grandori R.;Brocca S.;Alberghina L.
1993

Abstract

Lipases (Lip) hydrolyze triglycerides into fatty acids and glycerol. Lip produced by the yeast Candida cylindracea are encoded by multiple genomic sequences. We report the molecular cloning and characterization of three genes from this family. They encode putative mature 57-kDa proteins of 534 amino acids (aa). To date, five Lip-encoding genomic sequences from C. cylindracea have been characterized in our laboratory. The five deduced aa sequences share an overall homology of 80%. These sequences have been aligned with each other and with those of homologous enzymes, the Lip from the mould Geotrichum candidum and the acetylcholinesterase from Torpedo californica, whose three-dimensional structures have been solved by X-ray analysis. The C. cylindracea Lip appear to have a structural organization similar to that described for both enzymes. © 1993.
Articolo in rivista - Articolo scientifico
evolution; fungal extracellular lipase; gene structure; homology; Yeast
English
45
55
11
Lotti, M., Grandori, R., Fusetti, F., Longhi, S., Brocca, S., Tramontane, A., et al. (1993). Cloning and analysis of Candida cylindracea lipase sequences. GENE, 124(1), 45-55 [10.1016/0378-1119(93)90760-Z].
Lotti, M; Grandori, R; Fusetti, F; Longhi, S; Brocca, S; Tramontane, A; Alberghina, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/259051
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