Actin was localized in the four subacrosomal bulges (Camatini et al., Eur. J. Cell Biol. 45:276-281, 1987), which characterize the sperm head of rabbit spermatozoa (Phillips, J. Ultrastruct. Res. 38:591-604, 1972), and in the postacrosomal region (Welch and O'Rand, Dev. Biol. 109:411-417, 1985; Camatini et al., Eur. J. Cell Biol. 45:276-281, 1987). Specific antibodies and indirect immunogold labelling on testis Lowicryl K4M sections and spermatozoa cryosections were used to study the distribution of calmodulin and a spectrin-like protein. This protein was also present close to the shaping membranes of the head. The results presented suggest a membrane-cytoskeletal role of actin, spectrin, and calmodulin.

Camatini, M., Colombo, A., & Bonfanti, P. (1991). Identification of spectrin and calmodulin in rabbit spermiogenesis and spermatozoa. MOLECULAR REPRODUCTION AND DEVELOPMENT, 28(1), 62-69 [10.1002/mrd.1080280110].

Identification of spectrin and calmodulin in rabbit spermiogenesis and spermatozoa

CAMATINI, MARINA CARLA;COLOMBO, ANITA EMILIA;BONFANTI, PATRIZIA
1991-01

Abstract

Actin was localized in the four subacrosomal bulges (Camatini et al., Eur. J. Cell Biol. 45:276-281, 1987), which characterize the sperm head of rabbit spermatozoa (Phillips, J. Ultrastruct. Res. 38:591-604, 1972), and in the postacrosomal region (Welch and O'Rand, Dev. Biol. 109:411-417, 1985; Camatini et al., Eur. J. Cell Biol. 45:276-281, 1987). Specific antibodies and indirect immunogold labelling on testis Lowicryl K4M sections and spermatozoa cryosections were used to study the distribution of calmodulin and a spectrin-like protein. This protein was also present close to the shaping membranes of the head. The results presented suggest a membrane-cytoskeletal role of actin, spectrin, and calmodulin.
Articolo in rivista - Articolo scientifico
Scientifica
Male; Spermatozoa; Fluorescent Antibody Technique; Microscopy, Immunoelectron; Calmodulin; Immunoblotting; Spectrin; Spermatogenesis; Animals; Rabbits
English
Camatini, M., Colombo, A., & Bonfanti, P. (1991). Identification of spectrin and calmodulin in rabbit spermiogenesis and spermatozoa. MOLECULAR REPRODUCTION AND DEVELOPMENT, 28(1), 62-69 [10.1002/mrd.1080280110].
Camatini, M; Colombo, A; Bonfanti, P
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/25794
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