Nanomaterials are attracting growing interest for their potential use in several applications as nanomedicine; therefore, the analysis of their potential toxic effects on various cellular models, including circulating blood cells, is mandatory. This study aimed to investigate the effect of three unrelated nanomaterials, namely nanoscale silica, multiwalled carbon nanotubes, and carbon black, on platelet activation and aggregation. We found that these nanomaterials stimulate some of the typical biochemical pathways involved in canonical platelet activation, such as the stimulation of phospholipase C and Rap1b, resulting in the integrin α(IIb)β(3)-mediated platelet aggregation, through a mechanism largely dependent on the release of the extracellular second messengers ADP and thromboxane A(2). Importantly, we found that doses of nanoparticles unable to trigger appreciable responses can synergize with subthreshold amounts of physiological agonists to mediate platelet aggregation, indicating that even small amounts of nanomaterials in the bloodstream might contribute to the development of thrombosis

Guidetti, G., Consonni, A., Cipolla, L., Mustarelli, P., Balduini, C., Torti, M. (2012). Nanoparticles induce platelet activation in vitro through stimulation of canonical signalling pathways. NANOMEDICINE, 8(8), 1329-1336 [10.1016/j.nano.2012.04.001].

Nanoparticles induce platelet activation in vitro through stimulation of canonical signalling pathways

Mustarelli, P;
2012

Abstract

Nanomaterials are attracting growing interest for their potential use in several applications as nanomedicine; therefore, the analysis of their potential toxic effects on various cellular models, including circulating blood cells, is mandatory. This study aimed to investigate the effect of three unrelated nanomaterials, namely nanoscale silica, multiwalled carbon nanotubes, and carbon black, on platelet activation and aggregation. We found that these nanomaterials stimulate some of the typical biochemical pathways involved in canonical platelet activation, such as the stimulation of phospholipase C and Rap1b, resulting in the integrin α(IIb)β(3)-mediated platelet aggregation, through a mechanism largely dependent on the release of the extracellular second messengers ADP and thromboxane A(2). Importantly, we found that doses of nanoparticles unable to trigger appreciable responses can synergize with subthreshold amounts of physiological agonists to mediate platelet aggregation, indicating that even small amounts of nanomaterials in the bloodstream might contribute to the development of thrombosis
Articolo in rivista - Articolo scientifico
Silica nanoparticles; Multi-walled carbon nanotubes; Carbon black; Platelet aggregation; Signal transduction
English
2012
8
8
1329
1336
reserved
Guidetti, G., Consonni, A., Cipolla, L., Mustarelli, P., Balduini, C., Torti, M. (2012). Nanoparticles induce platelet activation in vitro through stimulation of canonical signalling pathways. NANOMEDICINE, 8(8), 1329-1336 [10.1016/j.nano.2012.04.001].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/256965
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