The effect of ethylisopropyl-amiloride (EIPA) and phenamil on sodium uptake in renal brush border membrane vesicles from prehypertensive rats of the Milan strain (MHS) and their normotensive controls (MNS) was investigated. In the presence of both a membrane potential and a pH gradient a differential effect of EIPA and phenamil was evidenced between the two rat strains. In the absence of a pH gradient, but in the presence of a membrane potential, EIPA was about two-fold more potent than phenamil in inhibiting sodium transport in both rat strains, excluding the presence of epithelial sodium channels in our BBMV preparations. Taken together these results support the hypothesis that a structurally different Na+/H+ exchanger located on the brush border membrane may be involved in the increased tubular sodium reabsorption observed in vivo in hypertensive rats

Parenti, P., Ferrari, P., Ferrandi, M., Hanozet, G., Bianchi, G. (1992). Effect of amiloride analogues on sodium transport in renal brush border membrane vesicles from milan hypertensive rats. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 183(1), 55-61 [10.1016/0006-291X(92)91608-S].

Effect of amiloride analogues on sodium transport in renal brush border membrane vesicles from milan hypertensive rats

PARENTI, PAOLO;
1992

Abstract

The effect of ethylisopropyl-amiloride (EIPA) and phenamil on sodium uptake in renal brush border membrane vesicles from prehypertensive rats of the Milan strain (MHS) and their normotensive controls (MNS) was investigated. In the presence of both a membrane potential and a pH gradient a differential effect of EIPA and phenamil was evidenced between the two rat strains. In the absence of a pH gradient, but in the presence of a membrane potential, EIPA was about two-fold more potent than phenamil in inhibiting sodium transport in both rat strains, excluding the presence of epithelial sodium channels in our BBMV preparations. Taken together these results support the hypothesis that a structurally different Na+/H+ exchanger located on the brush border membrane may be involved in the increased tubular sodium reabsorption observed in vivo in hypertensive rats
Articolo in rivista - Articolo scientifico
Membrane Potentials; Rats, Mutant Strains; Rats; Animals; Amiloride; Hypertension; Sodium; Microvilli; Kidney; Biological Transport, Active
English
1992
183
1
55
61
none
Parenti, P., Ferrari, P., Ferrandi, M., Hanozet, G., Bianchi, G. (1992). Effect of amiloride analogues on sodium transport in renal brush border membrane vesicles from milan hypertensive rats. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 183(1), 55-61 [10.1016/0006-291X(92)91608-S].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/25520
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