Two by-products containing phenols and polysaccharides, a “pâté” (OP) from the extra virgin olive oil milling process and a decoction of pomegranate mesocarp (PM), were investigated for their effects on human microbiota using the SHIME® system. The ability of these products to modulate the microbial community was studied simulating a daily intake for nine days. Microbial functionality, investigated in terms of short chain fatty acids (SCFA) and NH4+, was stable during the treatment. A significant increase in Lactobacillaceae and Bifidobacteriaceae at nine days was induced by OP mainly in the proximal tract. Polyphenol metabolism indicated the formation of tyrosol from OP mainly in the distal tract, while urolithins C and A were produced from PM, identifying the human donor as a metabotype A. The results confirm the SHIME® system as a suitable in vitro tool to preliminarily investigate interactions between complex botanicals and human microbiota before undertaking more challenging human studies.

Giuliani, C., Marzorati, M., Daghio, M., Franzetti, A., Innocenti, M., de Wiele, T., et al. (2019). Effects of olive and pomegranate by-products on human microbiota: A study using the SHIME® in vitro simulator. MOLECULES, 24(20), 3791 [10.3390/molecules24203791].

Effects of olive and pomegranate by-products on human microbiota: A study using the SHIME® in vitro simulator

Franzetti A.;
2019

Abstract

Two by-products containing phenols and polysaccharides, a “pâté” (OP) from the extra virgin olive oil milling process and a decoction of pomegranate mesocarp (PM), were investigated for their effects on human microbiota using the SHIME® system. The ability of these products to modulate the microbial community was studied simulating a daily intake for nine days. Microbial functionality, investigated in terms of short chain fatty acids (SCFA) and NH4+, was stable during the treatment. A significant increase in Lactobacillaceae and Bifidobacteriaceae at nine days was induced by OP mainly in the proximal tract. Polyphenol metabolism indicated the formation of tyrosol from OP mainly in the distal tract, while urolithins C and A were produced from PM, identifying the human donor as a metabotype A. The results confirm the SHIME® system as a suitable in vitro tool to preliminarily investigate interactions between complex botanicals and human microbiota before undertaking more challenging human studies.
Articolo in rivista - Articolo scientifico
Ellagitannins; Food by-products; Human gut; Microbial community; Tyrosol;
English
21-ott-2019
2019
24
20
3791
3791
open
Giuliani, C., Marzorati, M., Daghio, M., Franzetti, A., Innocenti, M., de Wiele, T., et al. (2019). Effects of olive and pomegranate by-products on human microbiota: A study using the SHIME® in vitro simulator. MOLECULES, 24(20), 3791 [10.3390/molecules24203791].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/253935
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