Molecular imaging of separate but still incompletely resolved spots on high-performance thin-layer chromatography (HPTLC) plates is used for the direct analysis of porcine brain lipids by desorption electrospray ionization mass spectrometry (DESI-MS). Eight class-specific spots were imaged in the negative ion mode and shown to contain more than fifty lipids. A low lateral resolution of 400 × 400 ftra allowed simple, rapid, and incomplete separation to be combined with DESI imaging for the identification of many components of these extremely complex mixtures. In this work, tandem mass spectrometry (MS/MS) was also employed to confirm the identity of particular lipids directly on HPTIX plates. © 2010 American Chemical Society

Paglia, G., Ifa, D., Wu, C., Corso, G., Graham Cooks, R. (2010). Desorption electrospray ionization mass spectrometry analysis of lipids after two-dimensional high-performance thin-layer chromatography partial separation. ANALYTICAL CHEMISTRY, 82(5), 1744-1750 [10.1021/ac902325j].

Desorption electrospray ionization mass spectrometry analysis of lipids after two-dimensional high-performance thin-layer chromatography partial separation

Paglia G.
Primo
;
2010

Abstract

Molecular imaging of separate but still incompletely resolved spots on high-performance thin-layer chromatography (HPTLC) plates is used for the direct analysis of porcine brain lipids by desorption electrospray ionization mass spectrometry (DESI-MS). Eight class-specific spots were imaged in the negative ion mode and shown to contain more than fifty lipids. A low lateral resolution of 400 × 400 ftra allowed simple, rapid, and incomplete separation to be combined with DESI imaging for the identification of many components of these extremely complex mixtures. In this work, tandem mass spectrometry (MS/MS) was also employed to confirm the identity of particular lipids directly on HPTIX plates. © 2010 American Chemical Society
Articolo in rivista - Articolo scientifico
Lipids, Imaging, Mass Spectrometry, DESI, Ambient Ionization
English
2010
82
5
1744
1750
none
Paglia, G., Ifa, D., Wu, C., Corso, G., Graham Cooks, R. (2010). Desorption electrospray ionization mass spectrometry analysis of lipids after two-dimensional high-performance thin-layer chromatography partial separation. ANALYTICAL CHEMISTRY, 82(5), 1744-1750 [10.1021/ac902325j].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/244065
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