Airborne ultrafine particles (UFP) mainly derive from combustion sources (e.g., diesel exhaust particles—DEP), abrasion sources (non-exhaust particles) or from the unintentional release of engineered nanoparticles (e.g., metal oxide nanoparticles—NPs), determining human exposure to UFP mixtures. The aim of the present study was to analyse the combined in vitro effects of DEP and metal oxide NPs (ZnO, CuO) on human lung A549 cells. The mixtures and the relative single NPs (DEP, ZnO, CuO) were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and inductively coupled plasma-optic emission spectroscopy (ICP-OES). Cells were exposed for different times (3–72 h) to mixtures of standard DEP at a subcytotoxic concentration and ZnO and CuO at increasing concentrations. At the end of the exposure, the cytotoxicity was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and clonogenic tests, the pro-inflammatory potential was evaluated by interleukin-8 (IL-8) release and the cell morphology was investigated by fluorescence and transmission electron microscopy. The obtained results suggest that the presence of DEP may introduce new physico-chemical interactions able to increase the cytotoxicity of ZnO and to reduce that of CuO NPs.

Zerboni, A., Bengalli, R., Baeri, G., Fiandra, L., Catelani, T., Mantecca, P. (2019). Mixture effects of diesel exhaust and metal oxide nanoparticles in human lung A549 cells. NANOMATERIALS, 9(9) [10.3390/nano9091302].

Mixture effects of diesel exhaust and metal oxide nanoparticles in human lung A549 cells

ZERBONI, ALESSANDRA
Primo
;
BENGALLI, ROSSELLA DANIELA;FIANDRA, LUISA;CATELANI, TIZIANO;Mantecca, Paride
Ultimo
2019

Abstract

Airborne ultrafine particles (UFP) mainly derive from combustion sources (e.g., diesel exhaust particles—DEP), abrasion sources (non-exhaust particles) or from the unintentional release of engineered nanoparticles (e.g., metal oxide nanoparticles—NPs), determining human exposure to UFP mixtures. The aim of the present study was to analyse the combined in vitro effects of DEP and metal oxide NPs (ZnO, CuO) on human lung A549 cells. The mixtures and the relative single NPs (DEP, ZnO, CuO) were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and inductively coupled plasma-optic emission spectroscopy (ICP-OES). Cells were exposed for different times (3–72 h) to mixtures of standard DEP at a subcytotoxic concentration and ZnO and CuO at increasing concentrations. At the end of the exposure, the cytotoxicity was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and clonogenic tests, the pro-inflammatory potential was evaluated by interleukin-8 (IL-8) release and the cell morphology was investigated by fluorescence and transmission electron microscopy. The obtained results suggest that the presence of DEP may introduce new physico-chemical interactions able to increase the cytotoxicity of ZnO and to reduce that of CuO NPs.
Articolo in rivista - Articolo scientifico
cytotoxicity; diesel exhaust; metal oxide nanoparticles; mixtures; ultrafine particles
English
Zerboni, A., Bengalli, R., Baeri, G., Fiandra, L., Catelani, T., Mantecca, P. (2019). Mixture effects of diesel exhaust and metal oxide nanoparticles in human lung A549 cells. NANOMATERIALS, 9(9) [10.3390/nano9091302].
File in questo prodotto:
File Dimensione Formato  
nanomaterials-09-01302-v2.pdf

accesso aperto

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 3.17 MB
Formato Adobe PDF
3.17 MB Adobe PDF Visualizza/Apri
nanomaterials-09-01302-s001.pdf

accesso aperto

Tipologia di allegato: Other attachments
Dimensione 764.95 kB
Formato Adobe PDF
764.95 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/242637
Citazioni
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 6
Social impact