The present study applies for the first time as Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Imaging (MSI) on real thyroid Fine Needle Aspirations (FNAs) to test its possible complementary role in routine cytology in the diagnosis of thyroid nodules. The primary aim is to evaluate the potential employment of MALDI-MSI in cytopathology, using challenging samples such as needle washes. Firstly, we designed a statistical model based on the analysis of Regions of Interest (ROIs), according to the morphological triage performed by the pathologist. Successively, the capability of the model to predict the classification of the FNAs was validated in a different group of patients on ROI and pixel-by-pixel approach. Results are very promising and highlight the possibility to introduce MALDI-MSI as a complementary tool for the diagnostic characterization of thyroid nodules.

Capitoli, G., Piga, I., Galimberti, S., Leni, D., Pincelli, A., Garancini, M., et al. (2019). MALDI-MSI as a Complementary Diagnostic Tool in Cytopathology: A Pilot Study for the Characterization of Thyroid Nodules. CANCERS, 11(9) [10.3390/cancers11091377].

MALDI-MSI as a Complementary Diagnostic Tool in Cytopathology: A Pilot Study for the Characterization of Thyroid Nodules

Capitoli, Giulia;Piga, Isabella;Galimberti, Stefania;Clerici, Francesca;Brambilla, Virginia;Smith, Andrew;Magni, Fulvio;Pagni, Fabio
2019

Abstract

The present study applies for the first time as Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Imaging (MSI) on real thyroid Fine Needle Aspirations (FNAs) to test its possible complementary role in routine cytology in the diagnosis of thyroid nodules. The primary aim is to evaluate the potential employment of MALDI-MSI in cytopathology, using challenging samples such as needle washes. Firstly, we designed a statistical model based on the analysis of Regions of Interest (ROIs), according to the morphological triage performed by the pathologist. Successively, the capability of the model to predict the classification of the FNAs was validated in a different group of patients on ROI and pixel-by-pixel approach. Results are very promising and highlight the possibility to introduce MALDI-MSI as a complementary tool for the diagnostic characterization of thyroid nodules.
Articolo in rivista - Articolo scientifico
Fine Needle Aspiration; MALDI-MSI; proteomics; thyroid carcinoma
English
2019
11
9
1377
open
Capitoli, G., Piga, I., Galimberti, S., Leni, D., Pincelli, A., Garancini, M., et al. (2019). MALDI-MSI as a Complementary Diagnostic Tool in Cytopathology: A Pilot Study for the Characterization of Thyroid Nodules. CANCERS, 11(9) [10.3390/cancers11091377].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/242635
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