Incerasing in production and exposure to engineered nanoparticles (NPs), make necessary to acquire information about NP potential adverse health effects. Many studies, focused on NP toxicity, highlighted their cytotoxic potential but there is a still a lack of information about the biological mechanisms involved. The aim of this research is the comparison of cytotoxicity between two types of metal nanoxides (CuO and TiO2) on A549 cells. After physico-chemical characterization, NPs were administered to cells. Cell-particle interactions, membrane integrity, viability and oxidative stress were investigated. CuO exposure resulted in a significant reduction of cell viability, while no effects were observed after TiO2 exposure. Both NPs induced cell cycle alteration, with a significant increase in frequency of cells in G1 and G2/M phases for TiO2 and CuO respectively. Confocal microscopy detected NPs at different cellular levels, and TEM imaging highlighted their ability to be internalized as aggregates by phagocytic processes or even as small agglomerates free in the cytoplasm.

Moschini, E., Gualtieri, M., Gallinotti, D., Pezzolato, E., Fascio, U., Camatini, M., et al. (2010). Metal oxide nanoparticles induce cytotoxic effects on human lung epithelial cells A549. In Chemical Engineering Transactions (pp.29-34). Italian Association of Chemical Engineering - AIDIC [10.3303/CET1022004].

Metal oxide nanoparticles induce cytotoxic effects on human lung epithelial cells A549

MOSCHINI, ELISA;GUALTIERI, MAURIZIO;CAMATINI, MARINA CARLA;Mantecca, P.
2010

Abstract

Incerasing in production and exposure to engineered nanoparticles (NPs), make necessary to acquire information about NP potential adverse health effects. Many studies, focused on NP toxicity, highlighted their cytotoxic potential but there is a still a lack of information about the biological mechanisms involved. The aim of this research is the comparison of cytotoxicity between two types of metal nanoxides (CuO and TiO2) on A549 cells. After physico-chemical characterization, NPs were administered to cells. Cell-particle interactions, membrane integrity, viability and oxidative stress were investigated. CuO exposure resulted in a significant reduction of cell viability, while no effects were observed after TiO2 exposure. Both NPs induced cell cycle alteration, with a significant increase in frequency of cells in G1 and G2/M phases for TiO2 and CuO respectively. Confocal microscopy detected NPs at different cellular levels, and TEM imaging highlighted their ability to be internalized as aggregates by phagocytic processes or even as small agglomerates free in the cytoplasm.
paper
nanoparticles, epithelial, A549, toxicity, in vitro
English
Advanced Atmoshperic Aereosol Symposium
2010
Chemical Engineering Transactions
9788895608136
2010
22
29
34
http://www.aidic.it/aaas10/webpapers/64Moschini.pdf
none
Moschini, E., Gualtieri, M., Gallinotti, D., Pezzolato, E., Fascio, U., Camatini, M., et al. (2010). Metal oxide nanoparticles induce cytotoxic effects on human lung epithelial cells A549. In Chemical Engineering Transactions (pp.29-34). Italian Association of Chemical Engineering - AIDIC [10.3303/CET1022004].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/24180
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