The integration of vascular structures into in vitro cultured tissues provides realistic models of complex tissue-vascular interactions. Despite the incidence and impact of muscle-wasting disorders, advanced in vitro systems are still far from recapitulating the environmental complexity of skeletal muscle. Our model comprises differentiated human muscle fibers enveloped by a sheath of human muscle-derived fibroblasts and supported by a vascular network with mural-like cells. Here, we demonstrate the induction of muscle-specific endothelium and the self-organization of endomysial muscle fibroblasts mediated by endothelial cells. We use this model to mimic the fibrotic environment characterizing muscular dystrophies and to highlight key signatures of fibrosis that are neglected or underestimated in traditional 2D monocultures. Overall, this vascularized meso-scale cellular construct finely recapitulates the human skeletal muscle environment and provides an advanced solution for in vitro studies of muscle physiology and pathology

Bersini, S., Gilardi, M., Ugolini, G., Sansoni, V., Talò, G., Perego, S., et al. (2018). Engineering an Environment for the Study of Fibrosis: A 3D Human Muscle Model with Endothelium Specificity and Endomysium. CELL REPORTS, 25(13), 3858-3868.e4 [10.1016/j.celrep.2018.11.092].

Engineering an Environment for the Study of Fibrosis: A 3D Human Muscle Model with Endothelium Specificity and Endomysium

Gilardi, Mara;Sansoni, Veronica;Zanotti, Simona;Vanoni, Marco;
2018

Abstract

The integration of vascular structures into in vitro cultured tissues provides realistic models of complex tissue-vascular interactions. Despite the incidence and impact of muscle-wasting disorders, advanced in vitro systems are still far from recapitulating the environmental complexity of skeletal muscle. Our model comprises differentiated human muscle fibers enveloped by a sheath of human muscle-derived fibroblasts and supported by a vascular network with mural-like cells. Here, we demonstrate the induction of muscle-specific endothelium and the self-organization of endomysial muscle fibroblasts mediated by endothelial cells. We use this model to mimic the fibrotic environment characterizing muscular dystrophies and to highlight key signatures of fibrosis that are neglected or underestimated in traditional 2D monocultures. Overall, this vascularized meso-scale cellular construct finely recapitulates the human skeletal muscle environment and provides an advanced solution for in vitro studies of muscle physiology and pathology
Articolo in rivista - Articolo scientifico
3D vascularized muscle model; endothelial specificity; fibrosis; muscle environment;
3D vascularized muscle model; endothelial specificity; fibrosis; muscle environment; Biochemistry, Genetics and Molecular Biology (all)
English
2018
25
13
3858
3868.e4
partially_open
Bersini, S., Gilardi, M., Ugolini, G., Sansoni, V., Talò, G., Perego, S., et al. (2018). Engineering an Environment for the Study of Fibrosis: A 3D Human Muscle Model with Endothelium Specificity and Endomysium. CELL REPORTS, 25(13), 3858-3868.e4 [10.1016/j.celrep.2018.11.092].
File in questo prodotto:
File Dimensione Formato  
BersiniCellReports2018.pdf

accesso aperto

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 5.17 MB
Formato Adobe PDF
5.17 MB Adobe PDF Visualizza/Apri
Bersini et al Cell Reports.pdf

Solo gestori archivio

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 5.17 MB
Formato Adobe PDF
5.17 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/222497
Citazioni
  • Scopus 55
  • ???jsp.display-item.citation.isi??? 54
Social impact