Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute to tissue repair after myocardial infarction (MI) by a variety of humoural and cellular mechanisms. However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs). To investigate whether BM cells contribute to repopulate the Kit+ CSCs pool, we transplanted BM cells from transgenic mice, expressing green fluorescent protein under the control of Kit regulatory elements, into wild-type irradiated recipients. Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs. These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5. These findings indicate that, at least in conditions of local acute cardiac damage, BM cells can home into the heart and give rise to cells that share properties of resident Kit+ CSCs

Barile, L., Cerisoli, F., Frati, G., Gaetani, R., Chimenti, I., Forte, E., et al. (2011). Bone marrow-derived cells can acquire cardiac stem cell properties in damaged heart. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 15(1), 63-71 [10.1111/j.1582-4934.2009.00968.x].

Bone marrow-derived cells can acquire cardiac stem cell properties in damaged heart

BARILE, LUCIO;CASSINELLI, LETIZIA MARIA;ALTOMARE, CLAUDIA;OTTOLENGHI, SERGIO;
2011

Abstract

Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute to tissue repair after myocardial infarction (MI) by a variety of humoural and cellular mechanisms. However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs). To investigate whether BM cells contribute to repopulate the Kit+ CSCs pool, we transplanted BM cells from transgenic mice, expressing green fluorescent protein under the control of Kit regulatory elements, into wild-type irradiated recipients. Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs. These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5. These findings indicate that, at least in conditions of local acute cardiac damage, BM cells can home into the heart and give rise to cells that share properties of resident Kit+ CSCs
Articolo in rivista - Articolo scientifico
Animals; Heart Diseases; Cell Differentiation; Mice; Mice, Transgenic; Reverse Transcriptase Polymerase Chain Reaction; Bone Marrow Cells; RNA, Messenger; Animals, Newborn; Blotting, Western; Regeneration; Stem Cells; Myocytes, Cardiac; Female
English
2011
63
71
9
Barile, L., Cerisoli, F., Frati, G., Gaetani, R., Chimenti, I., Forte, E., et al. (2011). Bone marrow-derived cells can acquire cardiac stem cell properties in damaged heart. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 15(1), 63-71 [10.1111/j.1582-4934.2009.00968.x].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/22057
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