The skin is an extremely extended organ of the body and, due to this large surface, it is continuously exposed to microorganisms. Skin damage can easily lead to infections in the dermis which can, in turn, result in the dissemination of pathogens into the bloodstream. Understanding how the immune system fights infections at the very early stage and how the host can eliminate the pathogens is an important step to set the base for future therapeutic interventions. Here we describe a model of Candida albicans infection that can visualize the processes that occur early during an infection, including when the pathogen has passed the epithelial barrier, as well as the immune response elicited by the C. albicans invasion. We used this infection model to perform histological analyses that show the immune cells that infiltrate the skin as well as the presence and localization of the pathogen. Samples collected after the infection can be processed for RNA extraction.

Santus, W., Mingozzi, F., Vai, M., Granucci, F., & Zanoni, I. (2018). Deep dermal injection as a model of candida albicans skin infection for histological analyses. JOURNAL OF VISUALIZED EXPERIMENTS, 2018(136) [10.3791/57574].

Deep dermal injection as a model of candida albicans skin infection for histological analyses

Santus, W;Mingozzi, F;Vai, M;Granucci, F
;
Zanoni, I
2018

Abstract

The skin is an extremely extended organ of the body and, due to this large surface, it is continuously exposed to microorganisms. Skin damage can easily lead to infections in the dermis which can, in turn, result in the dissemination of pathogens into the bloodstream. Understanding how the immune system fights infections at the very early stage and how the host can eliminate the pathogens is an important step to set the base for future therapeutic interventions. Here we describe a model of Candida albicans infection that can visualize the processes that occur early during an infection, including when the pathogen has passed the epithelial barrier, as well as the immune response elicited by the C. albicans invasion. We used this infection model to perform histological analyses that show the immune cells that infiltrate the skin as well as the presence and localization of the pathogen. Samples collected after the infection can be processed for RNA extraction.
Articolo in rivista - Articolo scientifico
Candida albicans; Histology; Immune system; Immunohistochemistry; Immunology and Infection; Infection; Issue 136; Skin;
Candida albicans; Histology; Immune system; Immunohistochemistry; Immunology and Infection; Infection; Issue 136; Skin; Animals; Candida albicans; Candidiasis; Dermis; Humans; Immunohistochemistry; Injections; Mice; Skin; Neuroscience (all); Chemical Engineering (all); Biochemistry, Genetics and Molecular Biology (all); Immunology and Microbiology (all)
English
Santus, W., Mingozzi, F., Vai, M., Granucci, F., & Zanoni, I. (2018). Deep dermal injection as a model of candida albicans skin infection for histological analyses. JOURNAL OF VISUALIZED EXPERIMENTS, 2018(136) [10.3791/57574].
Santus, W; Mingozzi, F; Vai, M; Granucci, F; Zanoni, I
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/219467
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