The final stages of restriction to the T cell lineage occur in the thymus after the entry of thymus-seeding progenitors (TSPs). The identity and lineage potential of TSPs remains unclear. Because the first embryonic TSPs enter a non-vascularized thymic rudiment, we were able to directly image and establish the functional and molecular properties of embryonic thymopoiesis-initiating progenitors (T-IPs) before their entry into the thymus and activation of Notch signaling. T-IPs did not include multipotent stem cells or molecular evidence of T cell-restricted progenitors. Instead, single-cell molecular and functional analysis demonstrated that most fetal T-IPs expressed genes of and had the potential to develop into lymphoid as well as myeloid components of the immune system. Moreover, studies of embryos deficient in the transcriptional regulator RBPJ demonstrated that canonical Notch signaling was not involved in pre-thymic restriction to the T cell lineage or the migration of T-IPs.

Luis, T., Luc, S., Mizukami, T., Boukarabila, H., Thongjuea, S., Woll, P., et al. (2016). Initial seeding of the embryonic thymus by immune-restricted lympho-myeloid progenitors. NATURE IMMUNOLOGY, 17(12), 1424-1435 [10.1038/ni.3576].

Initial seeding of the embryonic thymus by immune-restricted lympho-myeloid progenitors

Azzoni, E
Membro del Collaboration Group
;
2016

Abstract

The final stages of restriction to the T cell lineage occur in the thymus after the entry of thymus-seeding progenitors (TSPs). The identity and lineage potential of TSPs remains unclear. Because the first embryonic TSPs enter a non-vascularized thymic rudiment, we were able to directly image and establish the functional and molecular properties of embryonic thymopoiesis-initiating progenitors (T-IPs) before their entry into the thymus and activation of Notch signaling. T-IPs did not include multipotent stem cells or molecular evidence of T cell-restricted progenitors. Instead, single-cell molecular and functional analysis demonstrated that most fetal T-IPs expressed genes of and had the potential to develop into lymphoid as well as myeloid components of the immune system. Moreover, studies of embryos deficient in the transcriptional regulator RBPJ demonstrated that canonical Notch signaling was not involved in pre-thymic restriction to the T cell lineage or the migration of T-IPs.
Articolo in rivista - Articolo scientifico
Animals; Cell Differentiation; Cell Lineage; Cell Movement; Cells, Cultured; Fetus; Gene Expression Regulation, Developmental; Immunoglobulin J Recombination Signal Sequence-Binding Protein; Lymphoid Progenitor Cells; Mice; Mice, Inbred C57BL; Mice, Transgenic; Myeloid Progenitor Cells; Receptors, Notch; Signal Transduction; T-Lymphocytes; Thymus Gland; Immunology
English
2016
17
12
1424
1435
reserved
Luis, T., Luc, S., Mizukami, T., Boukarabila, H., Thongjuea, S., Woll, P., et al. (2016). Initial seeding of the embryonic thymus by immune-restricted lympho-myeloid progenitors. NATURE IMMUNOLOGY, 17(12), 1424-1435 [10.1038/ni.3576].
File in questo prodotto:
File Dimensione Formato  
Nat Immunol 2016 Luis.pdf

Solo gestori archivio

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 3.53 MB
Formato Adobe PDF
3.53 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
ni.3576.pdf

Solo gestori archivio

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 2.51 MB
Formato Adobe PDF
2.51 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/218610
Citazioni
  • Scopus 43
  • ???jsp.display-item.citation.isi??? 38
Social impact