Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) chitinase A (ChiA) is a protein which promotes the final liquefaction of infected host larvae. The potential of this viral molecule as a new tool for insect control is explored here. The ChiA gene was isolated from the AcMNPV genome by PCR and expressed in E. coli. The recombinant protein, purified by affinity chromatography, showed both exo- and endo-chitinase activities and produced perforations on the peritrophic membrane (PM) of Bombyx mori larvae which increased in number and in size, in a dose-dependent manner. This structural alteration resulted into a significant increase of PM permeability to methylene blue and to the small neuropeptide proctolin. When the fifth instar larvae of B. mori were fed on a artificial diet supplemented with the recombinant ChiA, 100% mortality was observed at a dose of 1 μg/g of larval body weight (LW), while at sub-lethal doses of 0.56 μg/g LW, a reduced larval growth was recorded. These results indicate that AcMNPV-ChiA may offer interesting new opportunities for pest control. © 2004 Elsevier Ltd. All rights reserved

Rao, R., Fiandra, L., Giordana, B., De Eguileor, M., Congiu, T., Burlini, N., et al. (2004). AcMNPV ChiA protein disrupts the peritrophic membrane and alters midgut physiology of Bombyx mori larvae. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 34(11), 1205-1213 [10.1016/j.ibmb.2004.08.002].

AcMNPV ChiA protein disrupts the peritrophic membrane and alters midgut physiology of Bombyx mori larvae

Fiandra, Luisa
Co-primo
;
2004

Abstract

Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) chitinase A (ChiA) is a protein which promotes the final liquefaction of infected host larvae. The potential of this viral molecule as a new tool for insect control is explored here. The ChiA gene was isolated from the AcMNPV genome by PCR and expressed in E. coli. The recombinant protein, purified by affinity chromatography, showed both exo- and endo-chitinase activities and produced perforations on the peritrophic membrane (PM) of Bombyx mori larvae which increased in number and in size, in a dose-dependent manner. This structural alteration resulted into a significant increase of PM permeability to methylene blue and to the small neuropeptide proctolin. When the fifth instar larvae of B. mori were fed on a artificial diet supplemented with the recombinant ChiA, 100% mortality was observed at a dose of 1 μg/g of larval body weight (LW), while at sub-lethal doses of 0.56 μg/g LW, a reduced larval growth was recorded. These results indicate that AcMNPV-ChiA may offer interesting new opportunities for pest control. © 2004 Elsevier Ltd. All rights reserved
Articolo in rivista - Articolo scientifico
Bioinsecticides; Gut absorption; Peritrophic membrane; Permeability; Recombinant protein; Viral chitinase; Animals; Base Sequence; Bombyx; Chitinases; DNA, Viral; Digestive System; Digestive System Physiological Phenomena; Escherichia coli; Genome, Viral; Larva; Microscopy, Electron, Scanning; Nucleopolyhedrovirus; Pest Control, Biological; Recombinant Proteins; Insect Science; Biochemistry
English
2004
34
11
1205
1213
none
Rao, R., Fiandra, L., Giordana, B., De Eguileor, M., Congiu, T., Burlini, N., et al. (2004). AcMNPV ChiA protein disrupts the peritrophic membrane and alters midgut physiology of Bombyx mori larvae. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 34(11), 1205-1213 [10.1016/j.ibmb.2004.08.002].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/218185
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