The interaction of a line of human breast carcinoma cells (MCF7) with basement membrane components, particularly laminin, was altered by exposure of the cells to estrogen as well as by transfection of the cells with the v-Ha-ras oncogene. In both cases, the cells show a greater ability to attach to a laminin substrate, to migrate to laminin, to grow in the presence of a basement membrane matrix, and to cross barriers of reconstituted basement membrane. These responses were associated with an increase in the expression of laminin receptors. It is postulated that the increase in the invasive behavior of the cells treated with estrogen or transfected with v-Ha-ras is related to the increased number of laminin receptors and their interaction with laminin. Estrogen had no discernible effect on the v-Ha-ras transfected cells. It appears that in the MCF7 cells, the malignant phenotype is under hormonal control and that this control is bypassed after v-Ha-ras transfection

Albini, A., J., G., Kitten, G., Kleinman, H., Martin, G., A., V., et al. (1986). 17 Beta-estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cells interaction with basement membrane. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 83(21), 8182-8186 [10.1073/pnas.83.21.8182].

17 Beta-estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cells interaction with basement membrane

ALBINI A
;
1986

Abstract

The interaction of a line of human breast carcinoma cells (MCF7) with basement membrane components, particularly laminin, was altered by exposure of the cells to estrogen as well as by transfection of the cells with the v-Ha-ras oncogene. In both cases, the cells show a greater ability to attach to a laminin substrate, to migrate to laminin, to grow in the presence of a basement membrane matrix, and to cross barriers of reconstituted basement membrane. These responses were associated with an increase in the expression of laminin receptors. It is postulated that the increase in the invasive behavior of the cells treated with estrogen or transfected with v-Ha-ras is related to the increased number of laminin receptors and their interaction with laminin. Estrogen had no discernible effect on the v-Ha-ras transfected cells. It appears that in the MCF7 cells, the malignant phenotype is under hormonal control and that this control is bypassed after v-Ha-ras transfection
Articolo in rivista - Articolo scientifico
Basement Membrane/*pathology Breast Neoplasms/pathology Cell Adhesion Cell Line Chemotaxis Estradiol/*pharmacology Female Fibronectins/pharmacology Human Laminin/metabolism/pharmacology *Neoplasm Invasiveness *Oncogenes Receptors, Immunologic/analysis Receptors, Laminin *Transfection
English
1986
83
21
8182
8186
none
Albini, A., J., G., Kitten, G., Kleinman, H., Martin, G., A., V., et al. (1986). 17 Beta-estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cells interaction with basement membrane. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 83(21), 8182-8186 [10.1073/pnas.83.21.8182].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/215335
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