Ageing induces a progressive morphological change and functional decline in muscles and in nerves. Light and electron microscopy, 2-D DIGE and MS, were applied to profile the qualitative and quantitative differences in the proteome and morphology of rat gastrocnemius muscle and sciatic nerve, in healthy 22-month-old rats. At muscle level, morphological changes are associated to fibre atrophy accompanied by myofibrillar loss and degeneration, disappearance of sarcomeres and sarcoplasmic reticulum dilatation, internal migration of nuclei, longitudinal fibre splitting, increment of subsarcolemmal mitochondria aggregates and increment of lipofuscin granules. Sciatic nerve shows myelin abnormalities like enfoldings, invaginations, onion bulbs, breakdowns and side axonal atrophy. Proteomic analysis identified changes correlated to morphological abnormalities in metabolic, contractile and cytoskeletal proteins, deregulation of iron homeostasis, change of Ca(2+) balance and stress response proteins, accompanied by a deregulation of myelin membrane adhesion protein and proteins regulating the neuronal caliber. By comparing proteomic results from the two tissues, 16 protein isoforms showed the same up and down regulation trend suggesting that there are changes implying a general process which may act as a signal event of degeneration. Only beta enolase and tropomyosin 1alpha were differentially expressed in the tissues

Capitanio, D., Vasso, M., Fania, C., Moriggi, M., Viganò, A., Procacci, P., et al. (2009). Comparative proteomic profile of rat sciatic nerve and gastrocnemius muscle tissues in ageing by 2-D DIGE. PROTEOMICS, 9(7), 2004-2020 [10.1002/pmic.200701162].

Comparative proteomic profile of rat sciatic nerve and gastrocnemius muscle tissues in ageing by 2-D DIGE

Fania, Chiara;
2009

Abstract

Ageing induces a progressive morphological change and functional decline in muscles and in nerves. Light and electron microscopy, 2-D DIGE and MS, were applied to profile the qualitative and quantitative differences in the proteome and morphology of rat gastrocnemius muscle and sciatic nerve, in healthy 22-month-old rats. At muscle level, morphological changes are associated to fibre atrophy accompanied by myofibrillar loss and degeneration, disappearance of sarcomeres and sarcoplasmic reticulum dilatation, internal migration of nuclei, longitudinal fibre splitting, increment of subsarcolemmal mitochondria aggregates and increment of lipofuscin granules. Sciatic nerve shows myelin abnormalities like enfoldings, invaginations, onion bulbs, breakdowns and side axonal atrophy. Proteomic analysis identified changes correlated to morphological abnormalities in metabolic, contractile and cytoskeletal proteins, deregulation of iron homeostasis, change of Ca(2+) balance and stress response proteins, accompanied by a deregulation of myelin membrane adhesion protein and proteins regulating the neuronal caliber. By comparing proteomic results from the two tissues, 16 protein isoforms showed the same up and down regulation trend suggesting that there are changes implying a general process which may act as a signal event of degeneration. Only beta enolase and tropomyosin 1alpha were differentially expressed in the tissues
Articolo in rivista - Articolo scientifico
Aging; Animals; Carrier Proteins; Contractile Proteins; Cytoskeletal Proteins; Electrophoresis, Gel, Two-Dimensional; Gene Expression Profiling; Male; Mass Spectrometry; Myosin Heavy Chains; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Signal Transduction; Gene Expression; Muscle, Skeletal; Sciatic Nerve
English
2009
9
7
2004
2020
none
Capitanio, D., Vasso, M., Fania, C., Moriggi, M., Viganò, A., Procacci, P., et al. (2009). Comparative proteomic profile of rat sciatic nerve and gastrocnemius muscle tissues in ageing by 2-D DIGE. PROTEOMICS, 9(7), 2004-2020 [10.1002/pmic.200701162].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/215272
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