The aim of this study was to investigate the lipid content and composition of rat cerebellar granule cells grown in the presence of ethanol (40, 55, or 80 mM) during in vitro differentiation. Quantitative analyses showed no effects of 40 mM ethanol, whereas a significant increase of total cholesterol was observed at 55 mM. Cells exposed to the highest ethanol dose (80 mM) were characterized by a higher sialidase activity, and by the modification of the ganglioside pattern and phospholipid fatty acid composition. The observed modifications were accompanied by changes of membrane anisotropy fluorescence assessed by the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene

Omodeo Salé, F., Pitto, M., Masserini, M., Palestini, P. (1995). Effects of chronic ethanol exposure on cultured cerebellar granule cells. MOLECULAR AND CHEMICAL NEUROPATHOLOGY, 26(2), 159-169 [10.1007/BF02815010].

Effects of chronic ethanol exposure on cultured cerebellar granule cells

PITTO, MARINA;MASSERINI, MASSIMO ERNESTO;PALESTINI, PAOLA NOVERINA ADA
1995

Abstract

The aim of this study was to investigate the lipid content and composition of rat cerebellar granule cells grown in the presence of ethanol (40, 55, or 80 mM) during in vitro differentiation. Quantitative analyses showed no effects of 40 mM ethanol, whereas a significant increase of total cholesterol was observed at 55 mM. Cells exposed to the highest ethanol dose (80 mM) were characterized by a higher sialidase activity, and by the modification of the ganglioside pattern and phospholipid fatty acid composition. The observed modifications were accompanied by changes of membrane anisotropy fluorescence assessed by the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene
Articolo in rivista - Articolo scientifico
Cerebellum; Time Factors; Cells, Cultured; Fluorescence Polarization; Neurons; Rats; Animals; Rats, Sprague-Dawley; Ethanol
English
159
169
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Omodeo Salé, F., Pitto, M., Masserini, M., Palestini, P. (1995). Effects of chronic ethanol exposure on cultured cerebellar granule cells. MOLECULAR AND CHEMICAL NEUROPATHOLOGY, 26(2), 159-169 [10.1007/BF02815010].
Omodeo Salé, F; Pitto, M; Masserini, M; Palestini, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/21481
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