The behaviour of a highly purified alpha-L-fucosidase (E.C. 3.2.1.51) extracted from octopus hepatopancreas was studied with phospholipid vesicles composed of phosphatidylcholine (PC) and phosphatidylserine (PS) containing the fucosylated ganglioside FucGM1, a potential natural substrate of the enzyme. The substrate recognition and hydrolysis take place only with PS/FucGM1 mixtures via an association process of the enzyme with the vesicles at acidic pH; the enzyme rapidly and stably binds to PS vesicles but not to PC vesicles. The data suggest that only the PS-associated enzyme is able to hydrolyse FucGM1 embedded in the same bilayer. The enzyme association with FucGM1/PS vesicles is a prerequisite for ganglioside hydrolysis but is followed by irreversible enzyme inactivation

Giuliani, A., Palestini, P., D'Aniello, A., & Masserini, M.E. (1993). Action of alpha-L-fucoside from Octopus vulgaris hepatopancreas on phospholipid vesicles containing the fucosylated ganglioside FucGM1. GLYCOCONJUGATE JOURNAL, 10(6), 447-452 [10.1007/BF00737965].

Action of alpha-L-fucoside from Octopus vulgaris hepatopancreas on phospholipid vesicles containing the fucosylated ganglioside FucGM1

PALESTINI, PAOLA NOVERINA ADA;MASSERINI, MASSIMO ERNESTO
1993

Abstract

The behaviour of a highly purified alpha-L-fucosidase (E.C. 3.2.1.51) extracted from octopus hepatopancreas was studied with phospholipid vesicles composed of phosphatidylcholine (PC) and phosphatidylserine (PS) containing the fucosylated ganglioside FucGM1, a potential natural substrate of the enzyme. The substrate recognition and hydrolysis take place only with PS/FucGM1 mixtures via an association process of the enzyme with the vesicles at acidic pH; the enzyme rapidly and stably binds to PS vesicles but not to PC vesicles. The data suggest that only the PS-associated enzyme is able to hydrolyse FucGM1 embedded in the same bilayer. The enzyme association with FucGM1/PS vesicles is a prerequisite for ganglioside hydrolysis but is followed by irreversible enzyme inactivation
Articolo in rivista - Articolo scientifico
Alpha-L-Fucosidase; Octopodiformes; Substrate Specificity; Lipid Bilayers; Phospholipids; Spectrometry, Fluorescence; Glycosides; Animals; G(M1) Ganglioside; Digestive System
English
447
452
6
Giuliani, A., Palestini, P., D'Aniello, A., & Masserini, M.E. (1993). Action of alpha-L-fucoside from Octopus vulgaris hepatopancreas on phospholipid vesicles containing the fucosylated ganglioside FucGM1. GLYCOCONJUGATE JOURNAL, 10(6), 447-452 [10.1007/BF00737965].
Giuliani, A; Palestini, P; D'Aniello, A; Masserini, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/21474
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