Previous studies have shown that sphingolipids may be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radiolabeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain. After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae. This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae

Fra, A., Masserini, M., Palestini, P., Sonnino, S., Simons, K. (1995). A photo-reactive derivative of ganglioside GM1 specifically cross-links VIP21-caveolin on the cell surface. FEBS LETTERS, 375(1-2), 11-14 [10.1016/0014-5793(95)95228-O].

A photo-reactive derivative of ganglioside GM1 specifically cross-links VIP21-caveolin on the cell surface

Masserini, ME;Palestini, PNA;
1995

Abstract

Previous studies have shown that sphingolipids may be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radiolabeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain. After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae. This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae
Articolo in rivista - Articolo scientifico
Caveolin 1; Cell Line; Dogs; Electrophoresis, Gel, Two-Dimensional; Membrane Proteins; Animals; Caveolins; Carrier Proteins; Humans; Tumor Cells, Cultured; Tritium; Cross-Linking Reagents; Molecular Sequence Data; Autoradiography; Carbohydrate Sequence; Kidney; Cell Membrane; G(M1) Ganglioside
English
11
14
4
Fra, A., Masserini, M., Palestini, P., Sonnino, S., Simons, K. (1995). A photo-reactive derivative of ganglioside GM1 specifically cross-links VIP21-caveolin on the cell surface. FEBS LETTERS, 375(1-2), 11-14 [10.1016/0014-5793(95)95228-O].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/21450
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