The exposure of GM1 molecular species present in the native ganglioside, carrying C18:1 or C20:1 long-chain bases (LCB), to Dactylium dendroides galactose oxidase was studied. When native GM1 (49.3% C18:1 and 50.7% C20:1 LCB, respectively), was inserted in dipalmitoylphosphatidylcholine vesicles and partially oxidized (10%), the proportion of C18:1 and C20:1 species in the oxidized GM1 was 59.6% and 40.4%, respectively, suggesting a preferential action of the enzyme on the shorter species. The Vmax of the enzyme was higher on C18:1 GM1 than on C20:1 GM1. The molecular species were affected without any preference after partial (10%) oxidation of GM1 incorporated in egg phosphatidylcholine vesicles or in micellar form. These data indicate that the exposure of the terminal galactose moiety of GM1 ganglioside to galactose oxidase is affected by the ganglioside ceramide composition as well as the phospholipid environment, that presumably determine the distribution (molecular dispersion, segregation) of the ganglioside within the membrane.

Palestini, P., Masserini, M., Tettamanti, G. (1994). Exposure to galactose oxidase of GM1 ganglioside molecular species embedded into phospholipid vesicles. FEBS LETTERS, 350(2-3), 219-222 [10.1016/0014-5793(94)00765-9].

Exposure to galactose oxidase of GM1 ganglioside molecular species embedded into phospholipid vesicles

PALESTINI, PAOLA NOVERINA ADA;MASSERINI, MASSIMO ERNESTO;
1994

Abstract

The exposure of GM1 molecular species present in the native ganglioside, carrying C18:1 or C20:1 long-chain bases (LCB), to Dactylium dendroides galactose oxidase was studied. When native GM1 (49.3% C18:1 and 50.7% C20:1 LCB, respectively), was inserted in dipalmitoylphosphatidylcholine vesicles and partially oxidized (10%), the proportion of C18:1 and C20:1 species in the oxidized GM1 was 59.6% and 40.4%, respectively, suggesting a preferential action of the enzyme on the shorter species. The Vmax of the enzyme was higher on C18:1 GM1 than on C20:1 GM1. The molecular species were affected without any preference after partial (10%) oxidation of GM1 incorporated in egg phosphatidylcholine vesicles or in micellar form. These data indicate that the exposure of the terminal galactose moiety of GM1 ganglioside to galactose oxidase is affected by the ganglioside ceramide composition as well as the phospholipid environment, that presumably determine the distribution (molecular dispersion, segregation) of the ganglioside within the membrane.
Articolo in rivista - Articolo scientifico
Kinetics; Structure-Activity Relationship; Cattle; Membrane Lipids; Liposomes; Animals; Galactose Oxidase; G(M1) Ganglioside; Brain Chemistry
English
219
222
Palestini, P., Masserini, M., Tettamanti, G. (1994). Exposure to galactose oxidase of GM1 ganglioside molecular species embedded into phospholipid vesicles. FEBS LETTERS, 350(2-3), 219-222 [10.1016/0014-5793(94)00765-9].
Palestini, P; Masserini, M; Tettamanti, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/21437
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